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      Correction: Evidence of inhibin/activin subunit betaC and betaE synthesis in normal human endometrial tissue

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          Abstract

          Correction We have previously demonstrated the expression of inhibin subunits in endometrial tissues and used β-actin primer pairs to perform a PCR analysis loading control [1]. However, instead of using the mentioned β-actin primer pairs from a commercial molecular biology supplier, we used the β-actin primer pair listed in Table 1. All depicted primer pairs in Table 1 were designed and established by our group, and synthesized by the supplier Biomers.net (Ulm, Germany). Table 1 Primer sequences and length of amplification products Forward primer (5' - 3') Backward primer (5' - 3') Length Inhibin-α CCGGCCATCCCAGCATACACGC GAGTTGAGCGTCGGGCTCTC 359 bp Inhibin-βA TGCCCTTGCTTTGGCTGAGA ACTTTGCCCACATGAAGCTTT 282 bp Inhibin-βB GGCGAGCGGCGACTCAACCTAGA CGTGTGGAAGGAGGAGGCAGAGC 333 bp Inhibin-βC GCAGCCCGGGTGAGAGTTGG ACTGCACCCACAGGCCTC 393 bp Inhibin-βE AGCCCTTCCTAGAGCTTAAG GCTGCAGCCACAGGCC 404 bp β-actin GGAGAAGCTGTGCTACGTCG CGCTCAGGAGGAGCAATGAT 366 bp bp = base pairs

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          Evidence of inhibin/activin subunit betaC and betaE synthesis in normal human endometrial tissue

          Background Inhibins are important regulators of the female reproductive system. Recently, two new inhibin subunits betaC and betaE have been described, although it is unclear if they are synthesized in normal human endometrium. Methods Samples of human endometrium were obtained from 82 premenopausal, non-pregnant patients undergoing gynecological surgery for benign diseases. Endometrium samples were classified according to anamnestic and histological dating into proliferative (day 1-14, n = 46), early secretory (day 15-22, n = 18) and late secretory phase (day 23-28, n = 18). Immunohistochemical analyses were performed with specific antibodies against inhibin alpha (n = 81) as well as inhibin betaA (n = 82), betaB (n = 82), betaC (n = 74) and betaE (n = 76) subunits. RT-PCR was performed for all inhibin subunits. Correlation was assessed with the Spearman factor to assess the relationship of inhibin-subunits expression within the different endometrial samples. Results The novel inhibin betaC and betaE subunits were found in normal human endometrium by immunohistochemical and molecular techniques. Inhibin alpha, betaA, betaB and betaE subunits showed a circadian expression pattern, being more abundant during the late secretory phase than during the proliferative phase. Additionally, a significant correlation between inhibin alpha and all inhibin beta subunits was observed. Conclusions The differential expression pattern of the betaC- and betaE-subunits in normal human endometrial tissue suggests that they function in endometrial maturation and blastocyst implantation. However, the precise role of these novel inhibin/activin subunits in human endometrium is unclear and warrants further investigation.
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            Author and article information

            Journal
            Reprod Biol Endocrinol
            Reproductive Biology and Endocrinology : RB&E
            BioMed Central
            1477-7827
            2011
            5 January 2011
            : 9
            : 1
            Affiliations
            [1 ]Ludwig-Maximilians-University Munich, 1st Department of Obstetrics and Gynecology, Maistrasse 11, 80337 Munich, Germany
            [2 ]Department of Obstetrics and Gynecology, Klinikum Neuperlach, Munich, Germany
            Article
            1477-7827-9-1
            10.1186/1477-7827-9-1
            3025943
            4a38f5b1-db2f-41bd-af0d-9704a8cbe8e6
            Copyright ©2011 Mylonas et al; licensee BioMed Central Ltd.

            This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

            History
            : 22 December 2010
            : 5 January 2011
            Categories
            Correction

            Human biology
            Human biology

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