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      Antibacterial interactions, anti-inflammatory and cytotoxic effects of four medicinal plant species

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          Abstract

          Background

          The constant emergence of antibiotic resistant species and the adverse side effects of synthetic drugs are threatening the efficacy of the drugs that are currently in use. This study was aimed at investigating the possible antibacterial interactions, anti-inflammatory and cytotoxic effects of selected medicinal plants based on their traditional usage.

          Methods

          The acetone extracts of four plant species were assessed independently and in combination for antibacterial activity using microdilution assay and the sum of the fractional inhibitory concentration (FIC) was calculated. The ability of Dombeya rotundifolia and Schkuhria pinnata extracts to inhibit the production of reactive oxygen species (ROS) in LPS induced RAW 264.7 macrophage cells was evaluated using Dichloro-dihydro-fluorescein diacetate (H 2DCF-DA) assay to determine anti-inflammatory potential and the toxicity on African green monkey kidney (Vero) cells was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.

          Results

          The antibacterial efficacies of the different combinations of Schkuhria pinnata (A), Commelina africana (B), Dombeya rotundifolia (C) and Elephantorrhiza elephantina (D) plants varied from combination to combination. Synergistic effects were only exhibited against P. aeruginosa, while the antagonistic effects were only observed against E. coli. Both S. pinnata and D. rotundifolia demonstrated anti-inflammatory potential by inhibiting the production of ROS in a dose dependant manner. The cytotoxicity of the plants (LC 50 values) ranged from < 25.0 to 466.1 μg/mL. S pinnata extract was the most toxic with the lowest LC 50 value of < 25.0 μg/mL.

          Conclusions

          The synergistic interaction observed indicates that combinational therapy may improve biological activity. This report highlights the anti-inflammatory potential of S. pinnata and D. rotundifolia; which could be exploited in the search for anti-inflammatory agents. However, the cytotoxicity of S. pinnata highlights the importance of using this plant with caution.

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          Most cited references36

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          A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria.

          J Eloff (1998)
          Agar diffusion techniques are used widely to assay plant extracts for antimicrobial activity, but there are problems associated with this technique. A micro-dilution technique was developed using 96-well microplates and tetrazolium salts to indicate bacterial growth. p-Iodonitrotetrazolium violet [0.2 mg/ml] gave better results than tetrazolium red or thiazolyl blue. The method is quick, worked well with Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli and with non-aqueous extracts from many different plants. The method gave reproducible results; required only 10-25 microliters of extract to determine minimal inhibitory concentrations, distinguished between microcidal and microstatic effects, and provided a permanent record of the results. Using S. aureus, and a Combretum molle extract, the technique was 32 times more sensitive than agar diffusion techniques and was not sensitive to culture age of the test organism up to 24 hours. The S. aureus culture could be stored up to 10 days in a cold room with little effect on the assay results. This method was useful in screening plants for antimicrobial activity and for the bioassay-guided isolation of antimicrobial compounds from plants. MIC values determined for sulfisoxazole, norfloxacin, gentamicin, and nitrofuratoin were similar to values indicated in the literature but values obtained with trimethroprim and ampicillin were higher with some bacteria.
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            Medicinal plants and antimicrobial activity.

            J.L. Ríos, M.C. Recio (2005)
            In the present paper, we analyze the past, present and future of medicinal plants, both as potential antimicrobial crude drugs as well as a source for natural compounds that act as new anti-infection agents. In the past few decades, the search for new anti-infection agents has occupied many research groups in the field of ethnopharmacology. When we reviewed the number of articles published on the antimicrobial activity of medicinal plants in PubMed during the period between 1966 and 1994, we found 115; however, in the following decade between 1995 and 2004, this number more than doubled to 307. In the studies themselves one finds a wide range of criteria. Many focus on determining the antimicrobial activity of plant extracts found in folk medicine, essential oils or isolated compounds such as alkaloids, flavonoids, sesquiterpene lactones, diterpenes, triterpenes or naphtoquinones, among others. Some of these compounds were isolated or obtained by bio-guided isolation after previously detecting antimicrobial activity on the part of the plant. A second block of studies focuses on the natural flora of a specific region or country; the third relevant group of papers is made up of specific studies of the activity of a plant or principle against a concrete pathological microorganism. Some general considerations must be established for the study of the antimicrobial activity of plant extracts, essential oils and the compounds isolated from them. Of utmost relevance is the definition of common parameters, such as plant material, techniques employed, growth medium and microorganisms tested.
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              Synergy research: approaching a new generation of phytopharmaceuticals.

              Hildebert Wagner (2011)
              The longstanding, successful use of herbal drug combinations in traditional medicine demands that we find a rationale for their comparative pharmacological and therapeutic superiority to isolated single constituents. The synergistic efficacy of these combinations can be evaluated and verified by Berenbaum's isobole method, followed by clinical studies performed in comparison with synthetic standard drugs. There are many examples of mono- and multi-extract combinations used presently, which exhibit synergistic efficiency based on multi-target mechanisms of action. Among the natural products, gallocatechins of green tea and curcuminoids of ginger are the presently favoured polyphenols for a possible future use in co-medication with antibiotics and standard anticancer drugs. The main targets were found to be COX 1+2, NF-κB, and membrane glycoproteins that belong to the ATP-binding cassette (ABC) transporter family. Copyright © 2010 Elsevier B.V. All rights reserved.
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                Author and article information

                Contributors
                Refilwe G. Kudumela: refilwekeith@gmail.com
                Lyndy J. McGaw: Lyndy.McGaw@up.ac.za
                Peter Masoko: Peter.Masoko@ul.ac.za
                Journal
                Journal ID (nlm-ta): BMC Complement Altern Med
                Journal ID (iso-abbrev): BMC Complement Altern Med
                Title: BMC Complementary and Alternative Medicine
                Publisher: BioMed Central (London )
                ISSN (Electronic): 1472-6882
                Publication date (Electronic): 3 July 2018
                Publication date PMC-release: 3 July 2018
                Publication date Collection: 2018
                Volume: 18
                Electronic Location Identifier: 199
                Affiliations
                [1 ]ISNI 0000 0001 2105 2799, GRID grid.411732.2, Department of Biochemistry, Microbiology and Biotechnology, Faculty of Science and Agriculture, , University of Limpopo, ; Turfloop Campus, Private Bag X1106, Sovenga, Limpopo 0727 South Africa
                [2 ]ISNI 0000 0001 2107 2298, GRID grid.49697.35, Department of Paraclinical Sciences, Phytomedicine Programme, Faculty of Veterinary Science, , University of Pretoria, ; Private Bag X04, Onderstepoort, 0110 South Africa
                Article
                Publisher ID: 2264
                DOI: 10.1186/s12906-018-2264-z
                PMC ID: 6029408
                PubMed ID: 29970064
                SO-VID: 5022cdaf-9010-4fd2-93b7-f62ccaca6e53
                Copyright © © The Author(s). 2018
                License:

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                Date received : 31 January 2018
                Date accepted : 22 June 2018
                Categories
                Subject: Research Article
                Custom metadata
                issue-copyright-statement © The Author(s) 2018

                ScienceOpen disciplines: Complementary & Alternative medicine
                Data availability:
                ScienceOpen disciplines: Complementary & Alternative medicine

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