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      Polycaprolactone Triol–Citrate Scaffolds Enriched with Human Platelet Releasates Promote Chondrogenic Phenotype and Cartilage Extracellular Matrix Formation

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          Abstract

          In this paper we report the differentiating properties of platelet-rich plasma releasates (PRPr) on human chondrocytes within elastomeric polycaprolactone triol–citrate (PCLT–CA) porous scaffold. Human-derived chondrocyte cellular content of glycosaminoglycans (GAGs) and total collagen were determined after seeding into PCLT–CA scaffold enriched with PRPr cells. Immunostaining and real time PCR was applied to evaluate the expression levels of chondrogenic and extracellular gene markers. Seeding of chondrocytes into PCLT–CA scaffold enriched with PRPr showed significant increase in total collagen and GAGs production compared with chondrocytes grown within control scaffold without PRPr cells. The mRNA levels of collagen II and SOX9 increased significantly while the upregulation in Cartilage Oligomeric Matrix Protein (COMP) expression was statistically insignificant. We also report the reduction of the expression levels of collagen I and III in chondrocytes as a consequence of proximity to PRPr cells within the scaffold. Interestingly, the pre-loading of PRPr caused an increase of expression levels of following extracellular matrix (ECM) proteins: fibronectin, laminin and integrin β over the period of 3 days. Overall, our results introduce the PCLT–CA elastomeric scaffold as a new system for cartilage tissue engineering. The method of PRPr cells loading prior to chondrocyte culture could be considered as a potential environment for cartilage tissue engineering as the differentiation and ECM formation is enhanced significantly.

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          Author and article information

          Contributors
          rothan@um.edu.my
          suhaeb@um.edu.my
          ivandjordjevich@hotmail.com
          m_golpich@yahoo.com
          rohana@um.edu.my
          simmratsingh@um.edu.my
          Journal
          Tissue Eng Regen Med
          Tissue Eng Regen Med
          Tissue Engineering and Regenerative Medicine
          Springer Singapore (Singapore )
          1738-2696
          2212-5469
          9 March 2017
          April 2017
          : 14
          : 2
          : 93-101
          Affiliations
          [1 ] ISNI 0000 0001 2308 5949, GRID grid.10347.31, Department of Molecular Medicine, Faculty of Medicine, , University of Malaya, ; 50603 Kuala Lumpur, Malaysia
          [2 ] ISNI 0000 0001 2308 5949, GRID grid.10347.31, Department of Orthopedic Surgery, Faculty of Medicine, , University of Malaya, ; 50603 Kuala Lumpur, Malaysia
          [3 ] ISNI 0000 0001 2224 0361, GRID grid.59025.3b, School of Materials Science and Engineering, , Nanyang Technological University, ; Singapore, 639798 Singapore
          [4 ] ISNI 0000 0004 1937 1557, GRID grid.412113.4, Department of Medicine, Faculty of Medicine, , University of Kebangsaan Malaysia, ; 56000 Cheras, Kuala Lumpur, Malaysia
          Article
          PMC6171579 PMC6171579 6171579 23
          10.1007/s13770-017-0023-8
          6171579
          30603466
          © The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht 2017
          Funding
          Funded by: FundRef http://dx.doi.org/10.13039/501100004386, Universiti Malaya;
          Award ID: RG377-15AFR
          Award Recipient :
          Categories
          Original Article
          Custom metadata
          © The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht 2017

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