Isolation and enzyme bioprospection of bacteria associated to Bruguiera cylindrica, a mangrove plant of North Sumatra, Indonesia

Highlights

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  Bruguiera cylindrica is a mangrove plant in North Sumatra with limited information on its microbial endophytes.

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  An enzyme bioprospection study was conducted revealing Vibrio alginolyticus as a prominent proteolytic strain.

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  Vibrio alginolyticus Jme3-20 produced a multitude of extracellular enzymes such as amylase, cellulase, chitinase, phosphatase, and urease.

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  This is the first report on finding Vibrio alginolyticus from mangrove area of North Sumatra.

Abstract

Mangrove-associated bacteria are of industrial interest due to their diverse and versatile enzyme properties. This study investigates the culturable bacteria from a wide range of habitat in a Bruguiera cylindrica mangrove ecosystem in North Sumatra. Screening of extracellular hydrolytic enzymes showed multiple potential traits in amylase, cellulase, chitinase, phosphatase, protease, and urease production by bacterial isolates. Molecular identification based on 16S rDNA region of a potential strain, Vibrio alginolyticus Jme3−20 is then reported as a newly proteolytic agent. The strain also showed a stable growth under salinity (NaCl) stress with considerable phosphate solubilization activities. Protease activity was enhanced by optimizing the 0.5 % (w/v) sucrose and soy peptone in the fermentation medium. SDS-PAGE and zymogram analysis showed the presence of a 35-kDa MW protease. Hence, our study revealed important insights into the bacterial diversity and activity in mangrove ecosystems, evidencing the importance of microbial exploration in this ecosystem.