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Detection of influenza A virus by RT-PCR and standard methods in experimental infection of Ducks.

The new microbiologica

Virus Cultivation, Cell Line, Chick Embryo, Cloaca, virology, Disease Models, Animal, Ducks, Animals, Influenza A virus, isolation & purification, Influenza in Birds, Poultry Diseases, Reverse Transcriptase Polymerase Chain Reaction, Swine

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      Abstract

      Cloacal swabs collected from mallard ducks (Anas platyrhynchos) experimentally infected with a H7N1 avian influenza strain were examined by Reverse Transcription Polymerase Chain Reaction to detect the influenza A virus. Reverse Transcription Polymerase Chain Reaction was compared with standard methods: inoculation of embryonated chicken eggs and inoculation of three established cell lines: Newborn Swine Kidney cells, Newborn Pig Trachea cells and Madine Darby Canine Kidney cells. Reverse Transcription Polymerase Chain Reaction was performed using a set of primers based on conserved regions of the matrix and nucleoprotein genes.

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      15782624

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