Erratum to: Development of a Multiplex-PCR probe system for the proper identification of Klebsiella variicola

Abstract

Erratum The original version of this article [1] unfortunately contained a mistake in Table 1. The primer sequence which corresponds to KmtnC-R in the table 1 was provided incorrectly. The correct nucleotide sequence is: KmtnC-R:GCATGGCCCAGGTGTTCAG An updated version of Table 1 has been provided below. Table 1 Amplification conditions, oligonucleotide combinations, sequence and amplification fragment of multiplex-PCR for K. variicola identification Amplification conditionsa Name of combination primers Shared unique genes, oligonucleotides and sequence (5′- 3′) of each bacterial specie K. pneumoniae Amplification fragment (bp) K. variicola Amplification fragment (bp) Klebsiella spp. Amplification fragment (bp) phosphohydrolase phosphoglycerate mutase phosphopentane phosphatase (mtnC) 1 M-PCR-1 KP888-F: AAGCAAGCCAGAACAGAAAG 888 KV770-F: TCCCGAGGTTCACATTTCC 449 KmtnC-F: CCGCCGACCTTATCACTAC 340 KP888-R: ACTTCGGTTTTATCCAGGTC KV770-R: AGCGGGTGAACGTCGATAC KmtnC-R: GCATGGCCCAGGTGTTCAG transferase (yphG) N-acetyltransferase phosphopentane phosphatase (mtnC) 1 M-PCR-2 KP878-F: ACCGATAACCAGCCTGACTT 878 KV1615-F: ACACAACATTTCAGGCGGCT 499 KmtnC-F: CCGCCGACCTTATCACTAC 340 KP878-R: CTTTCTTCTGCCCACTGTTG KV1615-R: GGGCGTGGCTTTTTTCATCG KmtnC-R: GCATGGCCCAGGTGTTCAG phosphohydrolase thiopurine S-methyltransferase phosphopentane phosphatase (mtnC) 2 M-PCR-3 KP888-F: AAGCAAGCCAGA ACAGAAAG 888 KV1000-F: CTGGGATGTGGCAATGGTG 438 KmtnC-F: CCGCCGACCTTATCACTAC 340 KP888-R: ACTTCGGTTTTATCCAGGTC KV1000-F: AAACTGCGCCTGCTGTATC KmtnC-R: GCATGGCCCAGGTGTTCAG aMultiplex-PCR conditions used under the oligonucleotides combinations. 1: 5pmol/reaction of K. variicola and Klebsiella spp, 25pmol/reaction of K. pneumoniae; 2: 25 pmol/reaction of K. pneumoniae, 5 pmol/reaction of K. variicola and 1 pmol/reaction of Klebsiella spp