Unique Roles of the Non-identical MCM Subunits in DNA Replication Licensing.

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

A family of six homologous subunits, Mcm2, -3, -4, -5, -6, and -7, each with its own unique features, forms the catalytic core of the eukaryotic replicative helicase. The necessity of six similar but non-identical subunits has been a mystery since its initial discovery. Recent cryo-EM structures of the Mcm2-7 (MCM) double hexamer, its precursors, and the origin recognition complex (ORC)-Cdc6-Cdt1-Mcm2-7 (OCCM) intermediate showed that each of these subunits plays a distinct role in orchestrating the assembly of the pre-replication complex (pre-RC) by ORC-Cdc6 and Cdt1.

Related collections

Author and article information

Journal

Journal ID (iso-abbrev): Mol. Cell

Title: Molecular cell

Publisher: Elsevier BV

ISSN (Electronic): 1097-4164

ISSN (Print): 1097-2765

Publication date (Electronic): Jul 20 2017

Volume: 67

Issue: 2

Affiliations

[1 ] Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Institute for Advanced Study, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. Electronic address: zhai@ust.hk.

[2 ] State Key Laboratory of Membrane Biology, Peking-Tsinghua Center for Life Sciences, School of Life Sciences, Peking University, Beijing, China.

[3 ] Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.

[4 ] State Key Laboratory of Membrane Biology, Peking-Tsinghua Center for Life Sciences, School of Life Sciences, Peking University, Beijing, China; Ministry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, China. Electronic address: gaon@pku.edu.cn.

[5 ] Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Department of Molecular Biology and Genetics, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA. Electronic address: biktye@ust.hk.

Article

Publisher Item ID: S1097-2765(17)30443-4

DOI: 10.1016/j.molcel.2017.06.016

PubMed ID: 28732205

SO-VID: 7c5d0198-dbf7-4faa-862d-822550335b91

History

Keywords: Mcm2–7,DNA replication initiation,ORC-Cdc6-Cdt1-MCM,cryo-EM structures,Acrobat Model for pre-RC assembly

Data availability:

Keywords: Mcm2–7, DNA replication initiation, ORC-Cdc6-Cdt1-MCM, cryo-EM structures, Acrobat Model for pre-RC assembly

Unique Roles of the Non-identical MCM Subunits in DNA Replication Licensing.

Read this article at

Abstract

Related collections

When did Helicobacter first colonise humans?

Author and article information

Journal

Affiliations

Article

History

Comments

Comment on this article

Similar content 200

Cited by 26