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      Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy

      ,

      Optics Letters

      The Optical Society

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          Abstract

          We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation point-spread function. In contrast to near-field scanning optical microscopy, this method can produce three-dimensional images of translucent specimens.

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          Most cited references 2

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          Improvement of lateral resolution in far-field fluorescence light microscopy by using two-photon excitation with offset beams

           Stefan Hell (1994)
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            Near-Field Optics: Imaging Single Molecules

             R Kopelman,  W Tan (1993)
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              Author and article information

              Journal
              OPLEDP
              Optics Letters
              Opt. Lett.
              The Optical Society
              0146-9592
              1539-4794
              1994
              1994
              June 01 1994
              June 01 1994
              : 19
              : 11
              : 780
              Article
              10.1364/OL.19.000780
              19844443
              © 1994

              Molecular medicine, Neurosciences

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