scREAD: A Single-Cell RNA-Seq Database for Alzheimer's Disease

Summary

Alzheimer's disease (AD) is a progressive neurodegenerative disorder of the brain and the most common form of dementia among the elderly. The single-cell RNA-sequencing (scRNA-Seq) and single-nucleus RNA-sequencing (snRNA-Seq) techniques are extremely useful for dissecting the function/dysfunction of highly heterogeneous cells in the brain at the single-cell level, and the corresponding data analyses can significantly improve our understanding of why particular cells are vulnerable in AD. We developed an integrated database named scREAD (single-cell RNA-Seq database for Alzheimer's disease), which is as far as we know the first database dedicated to the management of all the existing scRNA-Seq and snRNA-Seq data sets from the human postmortem brain tissue with AD and mouse models with AD pathology. scREAD provides comprehensive analysis results for 73 data sets from 10 brain regions, including control atlas construction, cell-type prediction, identification of differentially expressed genes, and identification of cell-type-specific regulons.

Graphical Abstract

Highlights

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  First-of-its-kind database dedicated to Alzheimer's disease sc/snRNA-Seq data sets

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  Control atlas and disease data sets construction for major cell types in the brain

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  User-friendly web server to provide comprehensive analysis interpretations

Abstract

Neuroscience; Bioinformatics; Biological Database