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      Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation.

      Proceedings of the National Academy of Sciences of the United States of America
      Proceedings of the National Academy of Sciences

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          Abstract

          Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images with the resolution and detail of standard histology without the use of exogenous stains. Imaging of intrinsic indicators within tissue, such as nicotinamide adenine dinucleotide, retinol, indoleamines, and collagen provides crucial information for physiology and pathology. The efficient application of multiphoton microscopy to intrinsic imaging requires knowledge of the nonlinear optical properties of specific cell and tissue components. Here we compile and demonstrate applications involving a range of intrinsic molecules and molecular assemblies that enable direct visualization of tissue morphology, cell metabolism, and disease states such as Alzheimer's disease and cancer.

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          Journal
          12756303
          165832
          10.1073/pnas.0832308100

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