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      Effect of salinity on growth and chemical composition of the diatom Thalassiosira weissflogii at three culture phases Translated title: Efecto de la salinidad en el crecimiento y composición química de la diatomea Thalassiosira weissflogii en tres fases de cultivo

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          Abstract

          The estuarine diatom Thalassiosira weissflogii (Fryxell & Hasle, 1977) has been widely used as live feed in aquaculture. The growth rate and biochemical composition of microalgae are highly influenced by environmental factors such as, light, salinity and nutrient availability. Salinity is difficult to control in some shrimp laboratories specialized in larvae production, because these laboratories depend upon the levels measured in estuaries or coastal lagoons, which are the water sources for larvae culture. The present study evaluated the effect of different salinities (25, 30, 35, 40, 45 and 50 psu), on the growth and chemical composition of T. weisflogii at three culture phases, under laboratory conditions. The highest growth rate and maximum cell density were found at 25 psu. Decrease in size and striking changes in morphology of the cells were observed at the higher salinities and drastic changes occurred at 50 psu. Protein and carbohydrate content were higher at low salinities (25 and 30 psu) during the stationary phase. The lipid production was higher at low salinities, but diminished as the phase changes occurred; in contrast, the lipid content was unaffected by the growth phase at higher salinities (≥ 35 psu). The higher growth rate and better biochemical composition were obtained at 25 and 30 psu.

          Translated abstract

          La diatomea estuarina Thalassiosira weissflogii (Fryxell & Hasle, 1977) ha sido utilizada como alimento vivo en acuacultura. La composición bioquímica del alimento vivo afecta la nutrición de los organismos durante su cultivo. La tasa de crecimiento y composición bioquímica de las microalgas están altamente influenciadas por factores ambientales como luz, salinidad y disponibilidad de nutrientes. En algunos laboratorios productores de larvas de camarón, es difícil controlar la salinidad, debido a que éstos dependen de los niveles presentes en estuarios o lagunas costeras, los cuales son la fuente de agua para el cultivo larvario. El presente estudio evaluó el efecto de diferentes salinidades (25, 30, 35, 40, 45 y 50 psu), sobre el crecimiento y la composición proximal de T. weissflogii en tres fases de cultivo, bajo condiciones de laboratorio. Las mayores tasas de crecimiento y la máxima densidad celular se obtuvieron a 25 psu. Se observó una reducción en tamaño y cambios en la morfología de las células a altas salinidades y los cambios drásticos ocurrieron a 50 psu. El contenido de proteínas y de carbohidratos fue más elevado a salinidades bajas (25 y 30 psu), durante la fase estacionaria de crecimiento. La producción de lípidos fue elevada a bajas salinidades y disminuyó a medida que cambiaba de fase; no se observó un efecto de las fases del cultivo sobre el contenido de lípidos en altas salinidades (≥ 35 psu). La mayor tasa de crecimiento y la mejor composición bioquímica se obtuvieron 25 y 30 psu.

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          Salt tolerance and salinity effects on plants: a review.

          Plants exposed to salt stress undergo changes in their environment. The ability of plants to tolerate salt is determined by multiple biochemical pathways that facilitate retention and/or acquisition of water, protect chloroplast functions, and maintain ion homeostasis. Essential pathways include those that lead to synthesis of osmotically active metabolites, specific proteins, and certain free radical scavenging enzymes that control ion and water flux and support scavenging of oxygen radicals or chaperones. The ability of plants to detoxify radicals under conditions of salt stress is probably the most critical requirement. Many salt-tolerant species accumulate methylated metabolites, which play crucial dual roles as osmoprotectants and as radical scavengers. Their synthesis is correlated with stress-induced enhancement of photorespiration. In this paper, plant responses to salinity stress are reviewed with emphasis on physiological, biochemical, and molecular mechanisms of salt tolerance. This review may help in interdisciplinary studies to assess the ecological significance of salt stress.
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            Effect of temperature on growth, chemical composition and fatty acid composition of tropical Australian microalgae grown in batch cultures

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              Biostatistical Analisis

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                Author and article information

                Journal
                lajar
                Latin american journal of aquatic research
                Lat. Am. J. Aquat. Res.
                Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar (Valparaíso, , Chile )
                0718-560X
                July 2012
                : 40
                : 2
                : 435-440
                Affiliations
                [03] Hermosillo Sonora orgnameCentro de Investigación en Alimentación y Desarrollo México
                [02] Sonora orgnameCentro de Estudios Superiores del Estado de Sonora México
                [01] Hermosillo Sonora orgnameUniversidad de Sonora orgdiv1Departamento de Investigaciones Científicas y Tecnológicas México
                Article
                S0718-560X2012000200018 S0718-560X(12)04000218
                10.3856/vol40-issue2-fulltext-18
                89ae2ac6-2d0e-4a29-a40d-9bace0b00b02

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 13 June 2012
                : 27 October 2011
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 22, Pages: 6
                Product

                SciELO Chile

                Categories
                Research Articles

                composición proximal,estuarine diatom,microalgae culture,proximate composition,diatomea marina,cultivo de microalgas

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