Frozen bovine preputial mucus as a suitable sample for the direct molecular diagnosis of Campylobacter fetus subsp. venerealis.

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Abstract

Long transportation times remain a challenge to the satisfactory diagnosis of Campylobacter fetus subsp. venerealis (Cfv). Here we demonstrated that samples of frozen bovine preputial mucus maintained at -20 °C for 10 days can be used as an alternative source for molecular diagnosis of Cfv. This approach will improve the analysis of this bacterium.

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Journal

Journal ID (iso-abbrev): J Microbiol Methods

Title: Journal of microbiological methods

Publisher: Elsevier BV

ISSN (Electronic): 1872-8359

ISSN (Print): 0167-7012

Publication date (Electronic): Dec 2020

Volume: 179

Affiliations

[1 ] Laboratório de Bacteriologia Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, 91540-000 Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, 91540-000 Porto Alegre, RS, Brazil.

[2 ] Laboratório de Bacteriologia Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, 91540-000 Porto Alegre, RS, Brazil.

[3 ] Departamento de Medicina Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, 91540-000 Porto Alegre, RS, Brazil.

[4 ] Veronica: Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul, BR290, km146, 92990-000, Eldorado do Sul, RS, Brazil.

[5 ] Virbac Saúde Animal, São Paulo, Av. Queiroz Filho, 1560 - Vila Leopoldina, 05317-000 São Paulo, SP, Brazil.

[6 ] Laboratório de Bacteriologia Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, 91540-000 Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, 91540-000 Porto Alegre, RS, Brazil. Electronic address: franciele.siqueira@ufrgs.br.

Article

Publisher Item ID: S0167-7012(20)30817-4

DOI: 10.1016/j.mimet.2020.106101

PubMed ID: 33181206

SO-VID: 8a79cf57-4e1d-4eaa-ab4e-b00c55cbb96f

History

Keywords: Sample conservation,Reproductive disease,Molecular diagnosis,Bulls,Bovine genital campylobacteriosis

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Keywords: Sample conservation, Reproductive disease, Molecular diagnosis, Bulls, Bovine genital campylobacteriosis

Frozen bovine preputial mucus as a suitable sample for the direct molecular diagnosis of Campylobacter fetus subsp. venerealis.

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