Establishing the Relationship between Complement Activation and Stimulation of Phagocyte Oxidative Metabolism in Hemodialyzed Patients: A Randomized Prospective Study

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

The present prospective study was conducted in order to establish the relationship between complement activation and stimulation of phagocyte oxidative metabolism observed in long-term hemodialysis (HD) patients during the early phase of dialysis with cellulosic membranes. Two groups of 10 randomized (HD) patients treated with cellulosic (Cuprophan, CUP) or synthetic polyacrilonitrile (PAN AN-69®) membranes were studied. Leukocyte counts, C3a antigen plasma concentration and whole blood basal and stimulated chemiluminescence (CL) production were determined in blood samples drawn from the fistula before dialysis (T₀) and from both the afferent and efferent lines of the dialyser at 15 min (T15) and at the end (Tend) of the dialysis session. This study confirms that, coincident with the nadir of leukopenia observed at T15, dialysis with CUP but not PAN membranes induces a marked rise in C3a antigen levels and profound alterations in whole blood CL production consisting of a dramatic increase in basal CL and a significant loss in CL response capacity to stimulating agents. It further demonstrates that a direct relationship exists between the variations in C3a antigen plasma levels and whole blood CL production observed in the CUP group of patients from T₀ to T15 (Δ15) of dialysis. This relationship is characterized by a positive correlation between Δ15 C3a and Δ15 basal CL levels in afferent and efferent lines, and a negative correlation between Δ15 C3a and Δ15 CL response capacity values in the efferent but not afferent line. In contrast, no significant correlation with the type of dialysis membrane could be demonstrated between the variations in polymorphonuclear neutrophil counts and C3a antigen levels. Altogether our results establish the relevance of C3a antigen and reactive oxygen intermediates as in vivo optimal biological parameters of dialysis membrane bioincompatibility and further document the possible role of reactive oxygen intermediates in HD-associated pathology.

Related collections

Author and article information

Journal

Journal ID (publisher-id): NEF

Journal ID (nlm-ta): Nephron

Journal ID (doi): 10.1159/issn.1660-8151

Title: Nephron

Publisher: S. Karger AG

ISSN (Print): 1660-8151

ISSN (Electronic): 2235-3186

Publication date Subscription-year: 1991

Publication date (Print): 1991

Publication date (Electronic): 11 December 2008

Volume: 59

Issue: 2

Pages: 279-285

Affiliations

^aINSERM U25 and CNRS UA 122, Département de Néphrologie, Hôpital Necker; ^bLaboratoire de Biostatistique, Hôpital Necker; ^cService d’Hémodialyse, C. H. Marines, Fleury-Mérogis; ^dINSERM U28, Hôpital Broussais, and ^eCinique de l’AURA, Paris, France

Article

Publisher ID: 186565 Other ID: Nephron 1991;59:279–285

DOI: 10.1159/000186565

PubMed ID: 1956490

SO-VID: 8b4c2993-0571-472b-918a-fd07fb213853

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date accepted : 18 December 1990

Page count

Pages: 7

Comments

Comment on this article

scite_

Cited by 11

See all cited by

- Version 1

Call for Papers: Green Renal Replacement Therapy: Caring for the Environment

Submit here before September 30, 2024