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      Detection of trace concentrations of S-nitrosothiols by means of a capacitive sensor

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          Abstract

          Small molecule S-nitrosothiols are a class of endogenous chemicals in the body, which have been implicated in a variety of biological functions. However, the labile nature of NO and the limits of current detection assays have made studying these molecules difficult. Here we present a method for detecting trace concentrations of S-nitrosothiols in biological fluids. Capacitive sensors when coupled to a semiconducting material represent a method for detecting trace quantities of a chemical in complex solutions. We have taken advantage of the semiconducting and chemical properties of polydopamine to construct a capacitive sensor and associated method of use, which specifically senses S-nitrosothiols in complex biological solutions.

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          Protein S-nitrosylation in health and disease: a current perspective.

          Protein S-nitrosylation constitutes a large part of the ubiquitous influence of nitric oxide on cellular signal transduction and accumulating evidence indicates important roles for S-nitrosylation both in normal physiology and in a broad spectrum of human diseases. Here we review recent findings that implicate S-nitrosylation in cardiovascular, pulmonary, musculoskeletal and neurological (dys)function, as well as in cancer. The emerging picture shows that, in many cases, pathophysiology correlates with hypo- or hyper-S-nitrosylation of specific protein targets rather than a general cellular insult due to loss of or enhanced nitric oxide synthase activity. In addition, it is increasingly evident that dysregulated S-nitrosylation can not only result from alterations in the expression, compartmentalization and/or activity of nitric oxide synthases, but can also reflect a contribution from denitrosylases, including prominently the S-nitrosoglutathione (GSNO)-metabolizing enzyme GSNO reductase. Finally, because exogenous mediators of protein S-nitrosylation or denitrosylation can substantially affect the development or progression of disease, potential therapeutic agents that modulate S-nitrosylation could well have broad clinical utility.
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            Oxidant-induced dopamine polymerization for multifunctional coatings

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              Microbial biosensors: a review.

              A microbial biosensor is an analytical device which integrates microorganism(s) with a physical transducer to generate a measurable signal proportional to the concentration of analytes. In recent years, a large number of microbial biosensors have been developed for environmental, food, and biomedical applications. Starting with the discussion of various sensing techniques commonly used in microbial biosensing, this review article concentrates on the summarization of the recent progress in the fabrication and application of microbial biosensors based on amperometry, potentiometry, conductometry, voltammetry, microbial fuel cell, fluorescence, bioluminescence, and colorimetry, respectively. Prospective strategies for the design of future microbial biosensors will also be discussed. Published by Elsevier B.V.
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                Author and article information

                Contributors
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: InvestigationRole: MethodologyRole: Project administrationRole: ResourcesRole: SoftwareRole: SupervisionRole: ValidationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: Data curationRole: Investigation
                Role: Data curationRole: Investigation
                Role: ConceptualizationRole: SupervisionRole: Writing – original draftRole: Writing – review & editing
                Role: ConceptualizationRole: Data curationRole: Funding acquisitionRole: ResourcesRole: Writing – original draftRole: Writing – review & editing
                Role: ConceptualizationRole: Data curationRole: InvestigationRole: SupervisionRole: Writing – original draftRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS ONE
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                26 October 2017
                2017
                : 12
                : 10
                : e0187149
                Affiliations
                [1 ] Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio, United States of America
                [2 ] Department of Anesthesia, University of Iowa, Iowa City, Iowa, United States of America
                [3 ] Rainbow Babies and Children’s Hospital, Cleveland, Ohio, United States of America
                Institute of Materials Science, GERMANY
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                http://orcid.org/0000-0001-7050-0666
                Article
                PONE-D-17-15398
                10.1371/journal.pone.0187149
                5658150
                29073241
                91d50db0-f20d-40d5-9663-a8c6af721daf
                © 2017 Seckler et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 20 April 2017
                : 14 October 2017
                Page count
                Figures: 7, Tables: 2, Pages: 21
                Funding
                Funded by: funder-id http://dx.doi.org/10.13039/100000002, National Institutes of Health;
                Award ID: 1 P01 HL128192
                Award Recipient :
                Funded by: funder-id http://dx.doi.org/10.13039/100000009, Foundation for the National Institutes of Health;
                Award ID: 1P01HL101871
                Award Recipient :
                Funded by: funder-id http://dx.doi.org/10.13039/100000009, Foundation for the National Institutes of Health;
                Award ID: 1T32HL125245
                Award Recipient :
                This study was supported by National Institutes of Health (NIH) Grants: 1 P01 HL128192, 1P01HL101871, 1T32HL125245.
                Categories
                Research Article
                Physical Sciences
                Chemistry
                Chemical Compounds
                Organic Compounds
                Amines
                Catecholamines
                Dopamine
                Physical Sciences
                Chemistry
                Organic Chemistry
                Organic Compounds
                Amines
                Catecholamines
                Dopamine
                Biology and Life Sciences
                Biochemistry
                Neurochemistry
                Neurotransmitters
                Biogenic Amines
                Catecholamines
                Dopamine
                Biology and Life Sciences
                Neuroscience
                Neurochemistry
                Neurotransmitters
                Biogenic Amines
                Catecholamines
                Dopamine
                Biology and Life Sciences
                Biochemistry
                Hormones
                Catecholamines
                Dopamine
                Physical Sciences
                Chemistry
                Chemical Compounds
                Organic Compounds
                Thiols
                Physical Sciences
                Chemistry
                Organic Chemistry
                Organic Compounds
                Thiols
                Physical Sciences
                Chemistry
                Electrochemistry
                Electrode Potentials
                Biology and Life Sciences
                Biochemistry
                Proteins
                Post-Translational Modification
                S-Nitrosylation
                Physical Sciences
                Chemistry
                Chemical Compounds
                Organic Compounds
                Formaldehyde
                Physical Sciences
                Chemistry
                Organic Chemistry
                Organic Compounds
                Formaldehyde
                Research and Analysis Methods
                Spectrum Analysis Techniques
                Absorption Spectroscopy
                Engineering and Technology
                Equipment
                Detectors
                Biosensors
                Physical Sciences
                Chemistry
                Chemical Compounds
                Organic Compounds
                Amino Acids
                Sulfur Containing Amino Acids
                Cysteine
                Physical Sciences
                Chemistry
                Organic Chemistry
                Organic Compounds
                Amino Acids
                Sulfur Containing Amino Acids
                Cysteine
                Biology and Life Sciences
                Biochemistry
                Proteins
                Amino Acids
                Sulfur Containing Amino Acids
                Cysteine
                Custom metadata
                All relevant data are within the paper and its Supporting Information files.

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