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      Large-field-of-view imaging by Multi-Pupil Adaptive Optics

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      Author(s): 1 , 1 , 1 , 1 , 2 , 3 , 4
      Publication date (Electronic):
      Journal: Nature methods

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          Abstract

          For in vivo deep imaging at high spatiotemporal resolutions, we developed Multi-Pupil Adaptive Optics (MPAO) which enables simultaneous wavefront correction over a large imaging field-of-view. The current implementation improves correction area by nine times over that of conventional methods. MPAO’s capability of spatially independent wavefront control further enables 3D nonplanar imaging. We applied MPAO to in vivo structural and functional imaging of biological dynamics in mammalian brain.

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          Focusing coherent light through opaque strongly scattering media

          I. Vellekoop, A P Mosk (2007)
          Article 0 comments Cited 247 times – based on 0 reviews     Review now
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            Is Open Access

            Thy1-GCaMP6 Transgenic Mice for Neuronal Population Imaging In Vivo

            Hod Dana, Tsai-Wen Chen, Amy Hu … (2014)
            Genetically-encoded calcium indicators (GECIs) facilitate imaging activity of genetically defined neuronal populations in vivo. The high intracellular GECI concentrations required for in vivo imaging are usually achieved by viral gene transfer using adeno-associated viruses. Transgenic expression of GECIs promises important advantages, including homogeneous, repeatable, and stable expression without the need for invasive virus injections. Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines for imaging in the visual cortex in vivo and compared their performance to mice injected with AAV expressing GCaMP6. Furthermore, we show that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice.
            Article 0 comments Cited 237 times – based on 0 reviews     Review now
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              Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo.

              Axel Nimmerjahn, Frank Kirchhoff, Jason N D Kerr … (2004)
              Glial cells have been identified as key signaling components in the brain; however, methods to investigate their structure and function in vivo have been lacking. Here, we describe a new, highly selective approach for labeling astrocytes in intact rodent neocortex that allows in vivo imaging using two-photon microscopy. The red fluorescent dye sulforhodamine 101 (SR101) was specifically taken up by protoplasmic astrocytes after brief exposure to the brain surface. Specificity was confirmed by immunohistochemistry. In addition, SR101 labeled enhanced green fluorescent protein (EGFP)-expressing astrocytes but not microglial cells in transgenic mice. We used SR101 labeling to quantify morphological characteristics of astrocytes and to visualize their close association with the cortical microvasculature. Furthermore, by combining this method with calcium indicator loading of cell populations, we demonstrated distinct calcium dynamics in astroglial and neuronal networks. We expect SR101 staining to become a principal tool for investigating astroglia in vivo.
              Article 0 comments Cited 231 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-journal-id): 101215604
                Journal ID (pubmed-jr-id): 32338
                Journal ID (nlm-ta): Nat Methods
                Journal ID (iso-abbrev): Nat. Methods
                Title: Nature methods
                ISSN (Print): 1548-7091
                ISSN (Electronic): 1548-7105
                Publication date Nihms-submitted: 20 April 2017
                Publication date (Electronic): 08 May 2017
                Publication date (Print): June 2017
                Publication date PMC-release: 08 November 2017
                Volume: 14
                Issue: 6
                Pages: 581-583
                Affiliations
                [1 ]School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana, USA
                [2 ]Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA
                [3 ]Integrated Imaging Cluster, Purdue University, West Lafayette, Indiana, USA
                [4 ]Bindley Bioscience Center, Purdue University, West Lafayette, Indiana, USA
                Author notes
                [* ]Correspondence and requests for materials should be addressed to Dr. Meng Cui, (+1) 765-494-5486, mengcui@ 123456purdue.edu
                [5]

                Current address: Department of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea

                [6]

                Current address: Department of Precision Instrument, Tsinghua University, Beijing, China

                [†]

                These authors contributed equally to this work.

                Article
                Manuscript ID: NIHMS868386
                DOI: 10.1038/nmeth.4290
                PMC ID: 5482233
                PubMed ID: 28481364
                SO-VID: 94fe6e7b-4f48-42b7-baa0-dd83c1fffc8c
                License:

                Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms

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                ScienceOpen disciplines: Life sciences

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