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      • Record: found
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      Retraction Note: Meeting Abstract: A post-hoc qualitative analysis of real time heads-up pollen counting versus traditional microscopy counting in the environmental exposure unit (EEU)

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          Abstract

          Retraction This meeting abstract [1], published in the Canadian Society of Allergy and Clinical Immunology and AllerGen Abstracts 2014 supplement, has been retracted from Allergy, Asthma & Clinical Immunology by the authors. The meeting abstract has previously been published in Journal of Allergy and Clinical Immunology [2] and was inadvertently submitted to Allergy, Asthma & Clinical Immunology as part of the supplement. The authors apologise for any inconvenience that this may have caused.

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          A post-hoc qualitative analysis of real time heads-up pollen counting versus traditional microscopy counting in the environmental exposure unit (EEU)

          Lisa Steacy, Terry Walker, Barnaby Hobsbawn … (2014)
          Background A custom digital imagery method for real time identification and counting of pollen was qualitatively evaluated in the Environmental Exposure Unit (EEU). Methods Airborne grass pollen was collected in the EEU via a Rotorod® impact sampler. The pollen grains on each sampling rod were counted using both traditional and heads-up microscopy. The heads-up technique incorporated a microscope camera to create an on-screen image of the sampling rod. Firstly, unique images were created by manually advancing the stage, without duplicating previously captured pollen grains. Well-defined, sharp images were obtained by fine focus and zoom combinations to enhance certainty and recognition speed. Secondly, using a custom application, each pollen grain was identified and counted on-screen by “point and click” or “screen touch”, simultaneously counting and permanently anchoring opaque dots to the pollen grain locations. Counts were stored in real time on a central database. Results Increased clarity of the pollen grains resulted in higher counting accuracy. Duplicate counting of pollen grains was eliminated by digitally labelling counted grains. Additional need for manual counting devices, commonly associated with mechanical and human errors, was eliminated. Error free counts can be obtained with increased speed, therefore, improving the overall efficiency of the process and the EEU system as a whole. Conclusions This validated heads-up counting technique will allow for an increased response time to changes in the EEU pollen levels. This advancement could also enhance pollen counting processes followed by others using direct microscopy pollen counting techniques.
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            A Post-Hoc Qualitative Analysis Of Real Time Heads-Up Pollen Counting Versus Traditional Microscopic Counting In The Environmental Exposure Unit (EEU)

            Lisa Steacy, Terry H Walker, Barnaby Hobsbawn … (2014)
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              Author and article information

              Contributors
              Lisa M Steacy: steacyl@kgh.kari.net
              Terry JB Walker: walkert1@kgh.kari.net
              Barnaby Hobsbawn: hobsbawb@KGH.KARI.NET
              Jenny Thiele: jenny.thiele@queensu.ca
              Anne K Ellis: ellisa@KGH.KARI.NET
              Journal
              Journal ID (nlm-ta): Allergy Asthma Clin Immunol
              Journal ID (iso-abbrev): Allergy Asthma Clin Immunol
              Title: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology
              Publisher: BioMed Central (London )
              ISSN (Print): 1710-1484
              ISSN (Electronic): 1710-1492
              Publication date (Electronic): 17 April 2015
              Publication date PMC-release: 17 April 2015
              Publication date Collection: 2015
              Volume: 11
              Issue: 1
              Electronic Location Identifier: 15
              Affiliations
              [ ]Allergy Research Unit, Kingston General Hospital, Ontario, Canada
              [ ]Department of Medicine, Queen’s University, Kingston, Ontario Canada
              Article
              Publisher ID: 79
              DOI: 10.1186/s13223-015-0079-8
              PMC ID: 4399747
              SO-VID: 9b1fab25-5ed8-470b-8fb2-a0db15f31d91
              Copyright © © Steacy et al.; licensee BioMed Central. 2015
              License:

              This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

              History
              Date received : 6 March 2015
              Date accepted : 6 March 2015
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              Subject: Retraction Note
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              issue-copyright-statement © The Author(s) 2015

              ScienceOpen disciplines: Immunology
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              ScienceOpen disciplines: Immunology

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