The development and validation of a fast and robust dried blood spot based lipid profiling method to study infant metabolism

Koulman, Albert; Prentice, Philippa; Wong, Max C. Y.; Matthews, Lee; Bond, Nicholas J.; Eiden, Michael; Griffin, Julian L; Dunger, David B.

doi:10.1007/s11306-014-0628-z

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The development and validation of a fast and robust dried blood spot based lipid profiling method to study infant metabolism

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Author(s): Albert Koulman , Philippa Prentice , Max C. Y. Wong , Lee Matthews , Nicholas J. Bond , Michael Eiden , Julian L. Griffin , David B. Dunger

Publication date (Electronic): 11 February 2014

Journal: Metabolomics

Publisher: Springer US

Keywords: Lipidomics, Dried blood spots, DIMS, FTMS, Infant lipid metabolism

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Abstract

Early life exposures and metabolic programming are associated with later disease risk. In particular lipid metabolism is thought to play a key role in the development of the metabolic syndrome and insulin resistance in later life. Investigative studies of metabolic programming are limited by the ethics and practicalities of sample collection in small infants. Dried blood spots on filter paper, derived from heel pricks are considered as the most suitable option for this age group. We validated a novel lipid profiling method, based on high resolution mass spectrometry to successfully determine the lipid composition of infants using dried blood spots. The spotting and air drying of blood on paper has noticeable effects on many of the lipids, leading to lipid oxidation and hydrolysis, which demand careful interpretation of the obtained data. We compared the lipid profiles from plasma or whole blood samples and the results from dried blood spots to determine if these revealed the same inter-subject differences. The results from dried blood spots were no less reproducible than other lipid profiling methods which required comparatively larger sample volumes. Therefore, lipid profiles obtained from dried blood spots can be successfully used to monitor infancy lipid metabolism and we show significant differences in the lipid metabolism of infants at age 3 versus 12 months.

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Author and article information

Contributors

Albert Koulman: +44 (0) 1223 426356 , +44 (0) 1223 4367515 , Albert.Koulman@mrc-hnr.cam.ac.uk

Journal

Journal ID (nlm-ta): Metabolomics

Journal ID (iso-abbrev): Metabolomics

Title: Metabolomics

Publisher: Springer US (Boston )

ISSN (Print): 1573-3882

ISSN (Electronic): 1573-3890

Publication date (Electronic): 11 February 2014

Publication date PMC-release: 11 February 2014

Publication date (Print): 2014

Volume: 10

Issue: 5

Pages: 1018-1025

Affiliations

[ ]Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, 120 Fulbourn Road, Cambridge, CB1 9NL UK

[ ]Department of Paediatrics, Addenbrooke’s Hospital, University of Cambridge, Cambridge, CB2 0QQ UK

Article

Publisher ID: 628

DOI: 10.1007/s11306-014-0628-z

PMC ID: 4145199

PubMed ID: 25177234

SO-VID: a5113d96-8cd1-42c8-9882-eefc63d35228

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.