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      The Cytoscan TM Model E-II, a New Reflectance Microscope for Intravital Microscopy: Comparison with the Standard Fluorescence Method


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          The Cytoscan<sup>TM</sup> Model E-II (Cytometrics Inc., Philadelphia, Pa., USA) is a newly developed instrument which functions as an intravital microscope and is small and easily portable. Through the use of orthogonal polarization spectral (OPS) imaging, the Cytoscan Model E-II delivers images of the microcirculation which are comparable to those achieved with intravital fluorescence videomicroscopy (IFM), but without the use of fluorescent dyes. The purpose of this study was to validate the Cytoscan Model E-II instrument against IFM. The experiments were carried out on striated muscle in the dorsal skinfold chamber of the awake Syrian hamster. The following parameters were measured in identical regions of interest in the same animal under baseline conditions and 0.5 and 2 h after a 4-hour period of pressure-induced ischemia: arteriolar diameter, venular diameter and venular red blood cell velocity. Bland-Altman plots showed good agreement between the two techniques for venular red blood cell velocity. As expected, arteriolar and venular diameters as measured by the Cytoscan were on average 5 µm smaller than the values from IFM, since the Cytoscan measures the red blood cell column width and IFM measures luminal diameter. Thus, OPS imaging can be used to make valid measurements of microvascular diameter and red blood cell velocity in tissues.

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          Sleep apnoea and nocturnal angina

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            Measurement uncertainties associated with the use of bright-field and fluorescence microscopy in the microcirculation.

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              Off-Line Analysis of Red Blood Cell Velocity in Renal Arterioles

              Videomicroscopic methods with off-line analysis of microcirculatory parameters by multifunctional computer-assisted image analysis systems have significant advantages for in vivo microvascular research. A limitation of these methods is, however, that red blood cell velocities (V RBC ) exceeding 2 mm/s cannot be measured using standard video framing rates. In the present study, a high-speed video camera, recording up to 600 frames per second, was incorporated in the set-up, and V RBC was measured off-line with the line-shift-diagram method. The aim of this study was to test the reproducibility and validity of the method using a high-speed video camera and to evaluate its applicability in vivo. V RBC were measured in arterioles of the split hydronephrotic kidney. The intra- and interindividual variability was small for V RBC below 40 mm/s. The validity of the method was tested using the mass conservation principle and found to be at least as good as that of the dual-slit photometric technique. The present approach extends the application of videomicroscopy coupled to image analysis systems to the analysis of high V RBC .

                Author and article information

                J Vasc Res
                Journal of Vascular Research
                S. Karger AG
                December 2000
                10 January 2001
                : 37
                : 6
                : 469-476
                Institute for Surgical Research, Klinikum Grosshadern, University of Munich, Munich, Germany
                54079 J Vasc Res 2000;37:469–476
                © 2000 S. Karger AG, Basel

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                Page count
                Figures: 5, Tables: 1, References: 22, Pages: 8
                Research Paper


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