For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
35
views
131
references
 Top references
cited by
24
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      183
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      RNA imaging in living cells – methods and applications

      review-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Numerous types of transcripts perform multiple functions in cells, and these functions are mainly facilitated by the interactions of the RNA with various proteins and other RNAs. Insight into the dynamics of RNA biosynthesis, processing and cellular activities is highly desirable because this knowledge will deepen our understanding of cell physiology and help explain the mechanisms of RNA-mediated pathologies. In this review, we discuss the live RNA imaging systems that have been developed to date. We highlight information on the design of these systems, briefly discuss their advantages and limitations and provide examples of their numerous applications in various organisms and cell types. We present a detailed examination of one application of RNA imaging systems: this application aims to explain the role of mutant transcripts in human disease pathogenesis caused by triplet repeat expansions. Thus, this review introduces live RNA imaging systems and provides a glimpse into their various applications.

          Related collections

          Most cited references131

          • Record: found
          • Abstract: found
          • Article: not found

          Molecular beacons: probes that fluoresce upon hybridization.

          S. Tyagi, F. R. Kramer (1996)
          We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization does not occur when the target contains a mismatched nucleotide or a deletion. The probes are particularly suited for monitoring the synthesis of specific nucleic acids in real time. When used in nucleic acid amplification assays, gene detection is homogeneous and sensitive, and can be carried out in a sealed tube. When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced.
          Article 0 comments Cited 599 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Myotonic dystrophy type 2 caused by a CCTG expansion in intron 1 of ZNF9.

            C Liquori (2001)
            Myotonic dystrophy (DM), the most common form of muscular dystrophy in adults, can be caused by a mutation on either chromosome 19q13 (DM1) or 3q21 (DM2/PROMM). DM1 is caused by a CTG expansion in the 3' untranslated region of the dystrophia myotonica-protein kinase gene (DMPK). Several mechanisms have been invoked to explain how this mutation, which does not alter the protein-coding portion of a gene, causes the specific constellation of clinical features characteristic of DM. We now report that DM2 is caused by a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the zinc finger protein 9 (ZNF9) gene. Parallels between these mutations indicate that microsatellite expansions in RNA can be pathogenic and cause the multisystemic features of DM1 and DM2.
            Article 0 comments Cited 279 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Recruitment of human muscleblind proteins to (CUG)(n) expansions associated with myotonic dystrophy.

              J. W. Miller (2000)
              Myotonic dystrophy (DM1) is an autosomal dominant neuromuscular disorder associated with a (CTG)(n) expansion in the 3'-untranslated region of the DM1 protein kinase (DMPK) gene. To explain disease pathogenesis, the RNA dominance model proposes that the DM1 mutation produces a gain-of-function at the RNA level in which CUG repeats form RNA hairpins that sequester nuclear factors required for proper muscle development and maintenance. Here, we identify the triplet repeat expansion (EXP) RNA-binding proteins as candidate sequestered factors. As predicted by the RNA dominance model, binding of the EXP proteins is specific for dsCUG RNAs and proportional to the size of the triplet repeat expansion. Remarkably, the EXP proteins are homologous to the Drosophila muscleblind proteins required for terminal differentiation of muscle and photoreceptor cells. EXP expression is also activated during mammalian myoblast differentiation, but the EXP proteins accumulate in nuclear foci in DM1 cells. We propose that DM1 disease is caused by aberrant recruitment of the EXP proteins to the DMPK transcript (CUG)(n) expansion.
              Article 0 comments Cited 271 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): RNA Biol
                Journal ID (iso-abbrev): RNA Biol
                Journal ID (pmc): KRNB
                Title: RNA Biology
                Publisher: Taylor & Francis
                ISSN (Print): 1547-6286
                ISSN (Electronic): 1555-8584
                Publication date Collection: August 2014
                Publication date (Electronic): 31 October 2014
                Volume: 11
                Issue: 8
                Pages: 1083-1095
                Affiliations
                Department of Molecular Biomedicine; Institute of Bioorganic Chemistry; Polish Academy of Sciences ; Poznan, Poland
                Author notes
                [* ]Correspondence to: Wlodzimierz J. Krzyzosiak; Email: wlodkrzy@ 123456ibch.poznan.pl
                Article
                Publisher ID: 977166
                DOI: 10.4161/rna.35506
                PMC ID: 4615301
                PubMed ID: 25483044
                SO-VID: ad7e59b0-7136-4768-8c2d-533d767bc2ac
                Copyright © © 2014 The Author(s). Published with license by Taylor & Francis Group, LLC© Martyna O Urbanek, Paulina Galka-Marciniak, Marta Olejniczak, and Wlodzimierz J Krzyzosiak
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License ( http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

                History
                Date received : 21 May 2014
                Date revision received : 24 July 2014
                Date accepted : 13 August 2014
                Page count
                Figures: 2, Tables: 4, References: 154, Pages: 13
                Categories
                Subject: Review

                ScienceOpen disciplines: Molecular biology
                Keywords: live-cell imaging systems,rna nuclear foci,rna localization,rna fluorescence imaging,triplet repeat diseases
                Data availability:
                ScienceOpen disciplines: Molecular biology
                Keywords: live-cell imaging systems, rna nuclear foci, rna localization, rna fluorescence imaging, triplet repeat diseases

                Comments

                Comment on this article

                scite_

                Similar content183

                See all similar

                Cited by23

                See all cited by

                Most referenced authors2,378

                See all reference authors