The protein hydrolysate, Primatone RL, is a cost-effective multiple growth promoter of mammalian cell culture in serum-containing and serum-free media and displays anti-apoptosis properties
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Abstract
The tryptic meat digest Primatone RL is a low-cost medium supplement of a complex
nature which serves as a source of amino acids, oligopeptides, iron salts, some lipids
and other trace low molecular weight substances. Its addition to mammalian and insect
cell culture media significantly improves the cell growth properties of many cell
lines. In this work the growth promoting effects of Primatone RL are described in
more detail using different mouse hybridomas, a mouse myeloma cell line, and human
promyelocytic leukemia HL-60 cells. The positive effects on cell growth induced by
Primatone were observed in the presence of serum but were even more pronounced in
serum-free culture. In addition the adaptation time from high serum to low (1%) or
serum-free growth in the presence of Primatone is also significantly reduced. Primatone
RL, when added to HL and DHI medium, improves cell growth under low serum or serum-free
conditions by increasing the maximum cell numbers and in particular the viability
of the culture. The observed decrease in cell death (apoptosis) induction leads to
a significant improvement in antibody (recombinant protein) production by increasing
the volumetric yields during long-term batch culture. The so-called anti-apoptotic
effects of Primatone RL for mouse hybridomas, which is concentration dependent, is
not fully understood.