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      Biotechnological formation of dairy flavor inducing δ-lactones from vegetable oil

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          Highlights

          • LAB effective in formation of dairy flavor inducing δ-lactones from grapeseed oil.

          • Sub-lethal heat stress significantly improved the δ-lactone production by LAB.

          • Higher lactone yield was observed in early stationary phase.

          • Pre-fermentation step positively influence lactone generation.

          • Insight into the development and optimization of a novel fermentation for δ-lactones.

          Abstract

          Agroscope Culture Collection was screened to identify bacterial strains effective in production of dairy flavor inducing lactones using grapeseed oil as a substrate. Lentilactobacillus parafarraginis FAM-1079, Lactococcus lactis subsp. lactis FAM-17918, and L. lactis subsp. lactis biovar diacetylactis FAM-22003 showed the most efficient formation of targeted δ-lactones. The application of sublethal heat stress significantly increased target lactone production. The most profound improvement was for L. lactis subsp. lactis biovar diacetylactis where δ-octadecalactone generation was improved by factor of 9. The pre-fermentation step as well as growth phase in which bacteria are harvested did not have a significant impact on lactones yield. The lactone production process from vegetable oil developed in this study offers a new way of developing a natural flavor ingredient for incorporation into plant-based products.

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          Most cited references39

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          UniProt: a worldwide hub of protein knowledge

          (2018)
          Abstract The UniProt Knowledgebase is a collection of sequences and annotations for over 120 million proteins across all branches of life. Detailed annotations extracted from the literature by expert curators have been collected for over half a million of these proteins. These annotations are supplemented by annotations provided by rule based automated systems, and those imported from other resources. In this article we describe significant updates that we have made over the last 2 years to the resource. We have greatly expanded the number of Reference Proteomes that we provide and in particular we have focussed on improving the number of viral Reference Proteomes. The UniProt website has been augmented with new data visualizations for the subcellular localization of proteins as well as their structure and interactions. UniProt resources are available under a CC-BY (4.0) license via the web at https://www.uniprot.org/.
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            The Pfam protein families database in 2019

            Abstract The last few years have witnessed significant changes in Pfam (https://pfam.xfam.org). The number of families has grown substantially to a total of 17,929 in release 32.0. New additions have been coupled with efforts to improve existing families, including refinement of domain boundaries, their classification into Pfam clans, as well as their functional annotation. We recently began to collaborate with the RepeatsDB resource to improve the definition of tandem repeat families within Pfam. We carried out a significant comparison to the structural classification database, namely the Evolutionary Classification of Protein Domains (ECOD) that led to the creation of 825 new families based on their set of uncharacterized families (EUFs). Furthermore, we also connected Pfam entries to the Sequence Ontology (SO) through mapping of the Pfam type definitions to SO terms. Since Pfam has many community contributors, we recently enabled the linking between authorship of all Pfam entries with the corresponding authors’ ORCID identifiers. This effectively permits authors to claim credit for their Pfam curation and link them to their ORCID record.
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              The importance of food naturalness for consumers: Results of a systematic review

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                Author and article information

                Contributors
                Journal
                Food Chem X
                Food Chem X
                Food Chemistry: X
                Elsevier
                2590-1575
                19 January 2022
                30 March 2022
                19 January 2022
                : 13
                : 100220
                Affiliations
                [a ]Agroscope, Schwarzenburgstrasse 161, 3003 Bern, Switzerland
                [b ]Nestlé Technology Centre Dairy, Nestlé Strasse 3, 3510 Konolfingen, Switzerland
                Author notes
                [* ]Corresponding author. ziahassan.bss@ 123456gmail.com
                Article
                S2590-1575(22)00018-9 100220
                10.1016/j.fochx.2022.100220
                9039933
                b2bf91fc-b444-4bc4-a874-6ae8f9de42c3
                © 2022 The Author(s)

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                : 23 June 2021
                : 5 December 2021
                : 14 January 2022
                Categories
                Research Article

                δ-c10, δ-decalactone,δ-c12, δ-dodecalactone,δ-c14, δ-tetradecalactone,δ-c18, δ-octadecalactone,hfa, hydroxy fatty acid,acc, agroscope culture collection,mep, mid-exponential phase,esp, early stationary phase,od, optical density,qt, quantifier ion,qf, qualifier ion,loq, limit of quantitation,ufa, unsaturated fatty acid,hs, head space,gc-o, gas-chromatography–olfactometry,lab, lactic acid bacteria,spe, solid phase extraction,ri, retention index,aroma-active lactones,vegetable oil,flavor generation,fermentation,heat stress,lactic acid bacteria

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