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Abstract

Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range.

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PubMed ID:: 24056873

PMC ID:: 3860264

DOI:: 10.1038/nmeth.2647

ScienceOpen disciplines: Chemistry

Keywords: Animals,Calcium Signaling,genetics,Cilia,metabolism,Mice,Signal Transduction

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ScienceOpen disciplines: Chemistry

Keywords: Animals, Calcium Signaling, genetics, Cilia, metabolism, Mice, Signal Transduction

Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.

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