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      The ABA-deficiency suppressor locus HAS2 encodes the PPR protein LOI1/MEF11 involved in mitochondrial RNA editing.

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          Abstract

          The hot ABA-deficiency suppressor2 (has2) mutation increases drought tolerance and the ABA sensitivity of stomata closure and seed germination. Here we report that the HAS2 locus encodes the mitochondrial editing factor11 (MEF11), also known as lovastatin insensitive1. has2/mef11 mutants exhibited phenotypes very similar to the ABA-hypersensitive mutant, hai1-1 pp2ca-1 hab1-1 abi1-2, which is impaired in four genes encoding type 2C protein phosphatases (PP2C) that act as upstream negative regulators of the ABA signaling cascade. Like pp2c, mef11 plants were more resistant to progressive water stress and seed germination was more sensitive to paclobutrazol (a gibberellin biosynthesis inhibitor) as well as mannitol and NaCl, compared with the wild-type plants. Phenotypic alterations in mef11 were associated with the lack of editing of transcripts for the mitochondrial cytochrome c maturation FN2 (ccmFN2) gene, which encodes a cytochrome c-heme lyase subunit involved in cytochrome c biogenesis. Although the abundance of electron transfer chain complexes was not affected, their dysfunction could be deduced from increased respiration and altered production of hydrogen peroxide and nitric oxide in mef11 seeds. As minor defects in mitochondrial respiration affect ABA signaling, this suggests an essential role for ABA in mitochondrial retrograde regulation.

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          Author and article information

          Journal
          Mol Plant
          Molecular plant
          Elsevier BV
          1752-9867
          1674-2052
          Apr 2015
          : 8
          : 4
          Affiliations
          [1 ] INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
          [2 ] CEA Saclay, IBiTec-S, CNRS UMR 8221, Serv Bioenerget Biol Struct & Mécanisme, F-91191 Gif Sur Yvette, France.
          [3 ] Université d'Angers, UMR IRHS 1345, INRA, Agrocampus Ouest, F-49045 Angers, France.
          [4 ] INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; Institut Jean-Pierre Bourgin, INRA, Bât 7, F-78026 Versailles Cedex, France. Electronic address: hakim.mireau@versailles.inra.fr.
          [5 ] INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; Institut Jean-Pierre Bourgin, INRA, Bât 2, F-78026 Versailles Cedex, France. Electronic address: annie.marion-poll@versailles.inra.fr.
          Article
          S1674-2052(14)00033-1
          10.1016/j.molp.2014.12.005
          25708384
          c2ce3953-946f-4b5a-8316-d2d89d7f054b
          History

          pentatricopeptide repeat protein,germination,abscisic acid,RNA editing,mitochondria,drought tolerance

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