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      A sex chromosomal restriction-fragment-length marker linked to melanoma-determining Tu loci in Xiphophorus.

      Genomics
      Animals, Chromosome Mapping, Cyprinodontiformes, genetics, DNA Restriction Enzymes, Fish Diseases, Melanoma, veterinary, Polymorphism, Genetic, Sex Chromosomes

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          Abstract

          In Xiphophorus, the causative genetic information for melanoma formation has been assigned by classical genetics to chromosomal loci, which are located on the sex chromosomes. In our attempts to molecularly clone these melanoma-determining loci, named Tu, we have looked for restriction-fragment-length markers (RFLMs) linked to the Tu loci. These RFLMs should be useful in obtaining a physical map of a Tu locus, which will aid in the cloning of the corresponding sequences. DNA samples from various Xiphophorus strains and hybrids including those bearing different Tu wild-type, deletion and translocation chromosomes, were screened for the presence of random RFLMs using homologous or heterologous sequences as hybridization probes. We find an EcoRI restriction fragment which shows limited crosshybridization to the v-erb B gene--but not representing the authentic c-erb B gene of Xiphophorus--to be polymorphic with respect to different sex chromosomes. Linkage analysis revealed that a 5-kb fragment is linked to the Tu-Sd locus on the X chromosome, a 7-kb fragment is linked to the Tu-Sr locus on the Y chromosome, both of Xiphophorus maculatus, and that a 12-kb fragment is linked to the Tu-Li locus on the X chromosome of Xiphophorus variatus. Using different chromosomal mutants this RFLM has been mapped to a frequent deletion/translocation breakpoint of the X chromosome, less than 0.3 cM apart from the Tu locus.

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          Author and article information

          Journal
          2841190
          1203452
          10.1093/genetics/119.3.679

          Chemistry
          Animals,Chromosome Mapping,Cyprinodontiformes,genetics,DNA Restriction Enzymes,Fish Diseases,Melanoma,veterinary,Polymorphism, Genetic,Sex Chromosomes

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