Age, sex, and mating status discrimination in the sand fly Lutzomyia longipalpis using near infra-red spectroscopy (NIRS)

Abstract

Background

Understanding aspects related to the physiology and capacity of vectors is essential for effectively controlling vector-borne diseases. The sand fly Lutzomyia longipalpis has great importance in medical entomology for disseminating Leishmania parasites, the causative agent of Leishmaniasis, one of the main neglected diseases listed by the World Health Organization (WHO). In this respect, it is necessary to evaluate the transmission potential of this species and the success of vector control interventions. Near-infrared spectroscopy (NIRS) has been used to estimate the age of mosquitoes in different conditions (laboratory, semi-field, and conservation), taxonomic analysis, and infection detection. However, no studies are using NIRS for sand flies.

Methods

In this study, we developed analytic models to estimate the age of L. longipalpis adults under laboratory conditions, identify their copulation state, and evaluate their gonotrophic cycle and diet.

Results

Sand flies were classified with an accuracy of 58–82% in 3 age groups and 82–92% when separating them into young (<8 days) or old (>8 days) insects. The classification between mated and non-mated sandflies was 98–100% accurate, while the percentage of hits of females that had already passed the first gonotrophic cycle was only 59%.

Conclusions

We consider the age and copula estimation results very promising, as they provide essential aspects of vector capacity assessment, which can be obtained quickly and at a lower cost with NIRS.

Graphical Abstract

Supplementary Information

The online version contains supplementary material available at 10.1186/s13071-023-06097-1.

Key message

Determining adult dipterans sand flies' age and mating status may be necessary for critical assessment of vector control interventions. Near-infrared spectroscopy (NIRS) is a fast, low-cost technique with many advantages compared to the traditional techniques that follow ovarian or cuticle development or pteridine content. In this work, we show for the first time to our knowledge that NIRS may be used for discrimination between young and old sand flies and mated or non-mated adults of both sexes in the Leishmania vector Lutzomyia longipalpis.

Supplementary Information

The online version contains supplementary material available at 10.1186/s13071-023-06097-1.