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      In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.

      Angewandte Chemie (International Ed. in English)

      Agrobacterium tumefaciens, metabolism, Amino Acids, chemistry, Bacillus subtilis, Bacteria, Benzoxazoles, Biosensing Techniques, Cell Wall, Coumarins, Escherichia coli, Fluorescent Dyes, Microscopy, Peptidoglycan, biosynthesis

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          Abstract

          Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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          Author and article information

          Journal
          23055266
          3589519
          10.1002/anie.201206749

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