Mitochondrial iron–sulfur clusters: Structure, function, and an emerging role in vascular biology

Abstract

Iron-sulfur (Fe–S) clusters are essential cofactors most commonly known for their role mediating electron transfer within the mitochondrial respiratory chain. The Fe–S cluster pathways that function within the respiratory complexes are highly conserved between bacteria and the mitochondria of eukaryotic cells. Within the electron transport chain, Fe–S clusters play a critical role in transporting electrons through Complexes I, II and III to cytochrome c, before subsequent transfer to molecular oxygen. Fe–S clusters are also among the binding sites of classical mitochondrial inhibitors, such as rotenone, and play an important role in the production of mitochondrial reactive oxygen species (ROS). Mitochondrial Fe–S clusters also play a critical role in the pathogenesis of disease. High levels of ROS produced at these sites can cause cell injury or death, however, when produced at low levels can serve as signaling molecules. For example, Ndufs2, a Complex I subunit containing an Fe–S center, N2, has recently been identified as a redox-sensitive oxygen sensor, mediating homeostatic oxygen-sensing in the pulmonary vasculature and carotid body. Fe–S clusters are emerging as transcriptionally-regulated mediators in disease and play a crucial role in normal physiology, offering potential new therapeutic targets for diseases including malaria, diabetes, and cancer.

Graphical abstract

Highlights

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  Fe–S clusters are essential cofactors, evolutionarily conserved across taxa.

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  Fe–S clusters mediate electron transfer in the mitochondrial respiratory complexes.

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  Fe–S clusters are implicated in ROS production by Complexes I, II and III.

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  Cluster N2 required for oxygen-sensing in the pulmonary arteries and carotid body.

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  Fe–S clusters emerging as novel therapeutic targets in the treatment of diseases.