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      Two-Photon Lithography of 3D Nanocomposite Piezoelectric Scaffolds for Cell Stimulation.

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          Abstract

          In this letter, we report on the fabrication, the characterization, and the in vitro testing of structures suitable for cell culturing, prepared through two-photon polymerization of a nanocomposite resist. More in details, commercially available Ormocomp has been doped with piezoelectric barium titanate nanoparticles, and bioinspired 3D structures resembling trabeculae of sponge bone have been fabricated. After an extensive characterization, preliminary in vitro testing demonstrated that both the topographical and the piezoelectric cues of these scaffolds are able to enhance the differentiation process of human SaOS-2 cells.

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          Author and article information

          Journal
          ACS Appl Mater Interfaces
          ACS applied materials & interfaces
          American Chemical Society (ACS)
          1944-8252
          1944-8244
          Nov 25 2015
          : 7
          : 46
          Affiliations
          [1 ] Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia , Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy.
          [2 ] The Biorobotics Institute, Scuola Superiore Sant'Anna , Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy.
          [3 ] Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia , Piazza San Silvestro 12, 56127 Pisa, Italy.
          [4 ] NEST, Scuola Normale Superiore , Piazza San Silvestro 12, 56127 Pisa, Italy.
          [5 ] CNR-IPCF, UOS Pisa , Largo Pontecorvo 3, 56127 Pisa, Italy.
          [6 ] Department of Mechanical and Aerospace Engineering, Politecnico di Torino , Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
          Article
          10.1021/acsami.5b08764
          26548588
          fd7bccca-5d94-4ac3-9c1c-2c5eccd10ffc
          History

          bone tissue engineering,direct laser writing,piezoelectric stimulation,two-photon lithography,barium titanate nanoparticles

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