The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes

Abstract

Plant HAK/KUP/KT family members function as plasma membrane (PM) H ⁺/K ⁺ symporters and may modulate chemiosmotically-driven polar auxin transport (PAT). Here, we show that inactivation of OsHAK5, a rice K ⁺ transporter gene, decreased rootward and shootward PAT, tiller number, and the length of both lateral roots and root hairs, while OsHAK5 overexpression increased PAT, tiller number, and root hair length, irrespective of the K ⁺ supply. Inhibitors of ATP-binding-cassette type-B transporters, NPA and BUM, abolished the OsHAK5-overexpression effect on PAT. The mechanistic basis of these changes included the OsHAK5-mediated decrease of transmembrane potential (depolarization), increase of extracellular pH, and increase of PM-ATPase activity. These findings highlight the dual roles of OsHAK5 in altering cellular chemiosmotic gradients (generated continuously by PM H ⁺-ATPase) and regulating ATP-dependent auxin transport. Both functions may underlie the prominent effect of OsHAK5 on rice architecture, which may be exploited in the future to increase crop yield via genetic manipulations.

Abstract

The potassium transporter OsHAK5 in rice acts as a convergence point between the developmental pathways of tillering, lateral root and root-hair growth, and the auxin transport pathway. This study reveals dual roles of OsHAK5 in altering cellular chemiosmotic gradients (generated continuously by plasma membrane H ⁺-ATPase) and regulating ATP-dependent auxin transport.