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      Supercritical fluid extracts of rosemary leaves exhibit potent anti-inflammation and anti-tumor effects.

      Bioscience, biotechnology, and biochemistry

      biosynthesis, Animals, Tumor Necrosis Factor-alpha, Temperature, chemistry, Rosmarinus, Pressure, Plant Leaves, pharmacology, economics, Plant Extracts, Nitric Oxide, Mice, Mass Spectrometry, Humans, Free Radical Scavengers, Chromatography, High Pressure Liquid, Chromans, drug effects, Cell Survival, Cell Line, Butylated Hydroxytoluene, Antineoplastic Agents, Anti-Inflammatory Agents

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          Abstract

          Supercritical fluid SF-CO2 treatment of Rosemarinus officinalis L. fresh leaves under optimum conditions (80 degrees C at 5,000 psi) yielded 5.3% of extract supercritical fluid extraction (SFE)-80, in which five major active principles were identified by liquid chromatography/mass spectrometry (LC/MS), viz., rosmarinic acid, carnosol, 12-methoxycarnosic acid, carnosic acid, and methyl carnosate. Total phenolic content was 155.8 mg/ gallic acid equivalent (GAE)/g in SFE-80, which showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging of 81.86% at 0.01 mg/ml. When treated in RAW 264.7, apparent dose-dependent NO inhibition occurred at dosages of 1.56 to 6.25 microg/ml, and more drastically at 12.5 and 25 microg/ml. At 0.5 to 5.0 microg/ml, SFE-80 exhibited dose-dependent viability suppression and significant tumor necrosis factor alpha (TNF-alpha) production in Hep 3B, whereas no effect was found in Chang liver cells. Furthermore, no effect was observed in RAW 264.7 at dosages of 3.13 to 25 microg/ml, indicating that SFE-80 exhibited a noncytotoxic character. Conclusively, rosemary can be considered an herbal anti-inflammatory and anti-tumor agent.

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          Author and article information

          Journal
          17827696
          10.1271/bbb.70199

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