Current models of embryological development focus on intracellular processes such as gene expression and protein networks, rather than on the complex relationship between subcellular processes and the collective cellular organization these processes support. We have explored this collective behavior in the context of neocortical development, by modeling the expansion of a small number of progenitor cells into a laminated cortex with layer and cell type specific projections. The developmental process is steered by a formal language analogous to genomic instructions, and takes place in a physically realistic three-dimensional environment. A common genome inserted into individual cells control their individual behaviors, and thereby gives rise to collective developmental sequences in a biologically plausible manner. The simulation begins with a single progenitor cell containing the artificial genome. This progenitor then gives rise through a lineage of offspring to distinct populations of neuronal precursors that migrate to form the cortical laminae. The precursors differentiate by extending dendrites and axons, which reproduce the experimentally determined branching patterns of a number of different neuronal cell types observed in the cat visual cortex. This result is the first comprehensive demonstration of the principles of self-construction whereby the cortical architecture develops. In addition, our model makes several testable predictions concerning cell migration and branching mechanisms.
The proper operation of the brain depends on the correct developmental wiring of billions of neurons. Understanding this process of living self-construction is crucial not only for biological explanation and medical therapy, but could also provide an entirely new approach to industrial fabrication. We are approaching this problem through detailed simulation of cortical development. We have previously presented a software package that allows for simulation of cellular growth in a 3D space that respects physical forces and diffusion of substances, as well as an instruction language for specifying biologically plausible ‘genetic codes’. Here we apply this novel formalism to understanding the principles of cortical development in the context of multiple, spatially distributed agents that communicate only by local metabolic messages.