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      Complete Genome Assembly of Staphylococcus epidermidis AmMS 205

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          Abstract

          Staphylococcus epidermidis causes a large number of catheter-related sepsis infections annually in the United States. We present the 2.54-Mbp complete genome assembly of reference strain S. epidermidis AmMS 205, including a single 37.7-kbp plasmid. The annotated assembly is available in GenBank under accession numbers CP009046 and CP009047.

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          Consed: a graphical tool for sequence finishing.

          Sequencing of large clones or small genomes is generally done by the shotgun approach (Anderson et al. 1982). This has two phases: (1) a shotgun phase in which a number of reads are generated from random subclones and assembled into contigs, followed by (2) a directed, or finishing phase in which the assembly is inspected for correctness and for various kinds of data anomalies (such as contaminant reads, unremoved vector sequence, and chimeric or deleted reads), additional data are collected to close gaps and resolve low quality regions, and editing is performed to correct assembly or base-calling errors. Finishing is currently a bottleneck in large-scale sequencing efforts, and throughput gains will depend both on reducing the need for human intervention and making it as efficient as possible. We have developed a finishing tool, consed, which attempts to implement these principles. A distinguishing feature relative to other programs is the use of error probabilities from our programs phred and phrap as an objective criterion to guide the entire finishing process. More information is available at http:// www.genome.washington.edu/consed/consed. html.
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            Solexa Ltd.

            Solexa Ltd is developing an integrated system, based on a breakthrough single molecule sequencing technology, to address a US$2 billion market that is expected to grow exponentially alongside and as a consequence of further technological enhancements. The system, software and consumables will initially be sold to research organizations, pharmaceutical companies and diagnostic companies that will sequence large regions of genomic DNA, including whole genomes, at costs several orders of magnitude below current levels. Solexa expects to launch its first product in 2006, and as it continues to make time and cost efficiencies, additional products will be launched into the expanding markets that will have broad applications in basic research through to healthcare management.
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              Staphylococcus epidermidis infections.

              The opportunistic human pathogen Staphylococcus epidermidis has become the most important cause of nosocomial infections in recent years. Its pathogenicity is mainly due to the ability to form biofilms on indwelling medical devices. In a biofilm, S. epidermidis is protected against attacks from the immune system and against antibiotic treatment, making S. epidermidis infections difficult to eradicate.
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                Author and article information

                Journal
                Genome Announc
                Genome Announc
                ga
                ga
                GA
                Genome Announcements
                American Society for Microbiology (1752 N St., N.W., Washington, DC )
                2169-8287
                6 November 2014
                Nov-Dec 2014
                : 2
                : 6
                : e01059-14
                Affiliations
                [a ]Los Alamos National Laboratory (LANL), Los Alamos, New Mexico, USA
                [b ]Diagnostic Systems Division (DSD), United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Maryland, USA
                [c ]Naval Medical Research Center (NMRC)-Frederick, Fort Detrick, Maryland, USA
                [d ]Henry M. Jackson Foundation, Bethesda, Maryland, USA
                [e ]United States Army Edgewood Chemical Biological Center (ECBC), Aberdeen Proving Ground, Maryland, USA
                Author notes
                Address correspondence to S. L. Johnson, shannonj@ 123456lanl.gov .
                [*]

                Present address: M. B. Scholz, Michigan State University, East Lansing, Michigan, USA.

                Author information
                http://orcid.org/0000-0002-3972-9208
                Article
                genomeA01059-14
                10.1128/genomeA.01059-14
                4223448
                25377697
                35a6b15f-aa51-4d9c-b374-9868dfc5db59
                Copyright © 2014 Davenport et al.

                This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

                History
                : 8 September 2014
                : 30 September 2014
                Page count
                Pages: 2
                Categories
                Prokaryotes
                Custom metadata
                November/December 2014
                free

                Genetics
                Genetics

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