Populations are often divided categorically into distinct racial/ethnic groups based on social rather than biological constructs. Genetic ancestry has been suggested as an alternative to this categorization. Herein, we typed over 450,000 CpG sites in whole blood of 573 individuals of diverse Hispanic origin who also had high-density genotype data. We found that both self-identified ethnicity and genetically determined ancestry were each significantly associated with methylation levels at 916 and 194 CpGs, respectively, and that shared genomic ancestry accounted for a median of 75.7% (IQR 45.8% to 92%) of the variance in methylation associated with ethnicity. There was a significant enrichment (p=4.2×10 -64) of ethnicity-associated sites amongst loci previously associated environmental exposures, particularly maternal smoking during pregnancy. We conclude that differential methylation between ethnic groups is partially explained by the shared genetic ancestry but that environmental factors not captured by ancestry significantly contribute to variation in methylation.
Whether a person develops a particular disease can depend on both genetic and environmental factors. Many studies have found that people of different races and ethnicities have different likelihoods of acquiring certain diseases. Race and ethnicity are social constructs; that is, they are not necessarily defined biologically. However, shared ancestry will produce genetic links between members of a group. In addition, members of an ethnic group often share a culture or environment that may influence their risk of disease. For example, the ‘Mediterranean diet’ inspired by the dietary habits of Southern Italians has been shown to reduce the risk of heart disease, diabetes and cancer.
The addition of chemical groups – such as methyl groups – to DNA strands can affect the activity of nearby genes. Methylation is controlled by both genetic and environmental factors, and altered patterns of DNA methylation are seen in some diseases. It is therefore an ideal biological process to study to determine how race/ethnicity and ancestry contribute to a person’s susceptibility to disease.
Galanter et al. have now studied the patterns of methylation found in the blood of 573 people from diverse Latino ethnic sub-groups. The different groups displayed significantly different patterns of methylation at hundreds of locations across the genome. Genetic ancestry explained approximately 75% of the variation in methylation between the sub-groups. In addition, the methylation patterns at DNA locations known to be affected by environmental exposures – for example, by exposure to tobacco while in the womb – were disproportionately likely to be methylated differently in different sub-groups.
Now that more is known about the relative effects of race/ethnicity and genetic ancestry on methylation, the next step is to apply this knowledge to disease processes. This will help us to better understand the source of health disparities across different groups of people.