365
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Simple combinations of lineage-determining transcription factors prime cis-regulatory elements required for macrophage and B cell identities.

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Genome-scale studies have revealed extensive, cell type-specific colocalization of transcription factors, but the mechanisms underlying this phenomenon remain poorly understood. Here, we demonstrate in macrophages and B cells that collaborative interactions of the common factor PU.1 with small sets of macrophage- or B cell lineage-determining transcription factors establish cell-specific binding sites that are associated with the majority of promoter-distal H3K4me1-marked genomic regions. PU.1 binding initiates nucleosome remodeling, followed by H3K4 monomethylation at large numbers of genomic regions associated with both broadly and specifically expressed genes. These locations serve as beacons for additional factors, exemplified by liver X receptors, which drive both cell-specific gene expression and signal-dependent responses. Together with analyses of transcription factor binding and H3K4me1 patterns in other cell types, these studies suggest that simple combinations of lineage-determining transcription factors can specify the genomic sites ultimately responsible for both cell identity and cell type-specific responses to diverse signaling inputs.

          Related collections

          Author and article information

          Journal
          Mol Cell
          Molecular cell
          Elsevier BV
          1097-4164
          1097-2765
          May 28 2010
          : 38
          : 4
          Affiliations
          [1 ] Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
          Article
          S1097-2765(10)00366-7 NIHMS206920
          10.1016/j.molcel.2010.05.004
          2898526
          20513432
          43d9042a-d1f3-4b1e-bf7d-41673c6a6005
          Copyright 2010 Elsevier Inc. All rights reserved.
          History

          Comments

          Comment on this article