Excitable RhoA dynamics drive pulsed contractions in the early C. elegans embryo

Abstract

Pulsed actomyosin contractility underlies many morphogenetic processes. Here, Michaux et al. show that, in early C. elegans embryos, pulsed contractions are generated by intrinsically excitable RhoA dynamics, involving fast autoactivation of RhoA and delayed negative feedback through local actin-dependent recruitment of the RhoGAPs RGA-3/4.

Abstract

Pulsed actomyosin contractility underlies diverse modes of tissue morphogenesis, but the underlying mechanisms remain poorly understood. Here, we combined quantitative imaging with genetic perturbations to identify a core mechanism for pulsed contractility in early Caenorhabditis elegans embryos. We show that pulsed accumulation of actomyosin is governed by local control of assembly and disassembly downstream of RhoA. Pulsed activation and inactivation of RhoA precede, respectively, the accumulation and disappearance of actomyosin and persist in the absence of Myosin II. We find that fast (likely indirect) autoactivation of RhoA drives pulse initiation, while delayed, F-actin–dependent accumulation of the RhoA GTPase-activating proteins RGA-3/4 provides negative feedback to terminate each pulse. A mathematical model, constrained by our data, suggests that this combination of feedbacks is tuned to generate locally excitable RhoA dynamics. We propose that excitable RhoA dynamics are a common driver for pulsed contractility that can be tuned or coupled differently to actomyosin dynamics to produce a diversity of morphogenetic outcomes.