Evidence for a hierarchical transcriptional circuit in Drosophila male germline involving testis-specific TAF and two gene-specific transcription factors, Mod and Acj6

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Abstract

<p class="first" id="P1">To analyse transcription factors involved in gene regulation by testis-specific TAF (tTAF), tTAF-dependent promoters were mapped and analysed <i>in silico</i>. Core promoters show decreased AT content, paucity of classical promoter motifs, and enrichment with translation control element CAAAATTY. Scanning of putative regulatory regions for known position frequency matrices identified nineteen transcription regulators possibly contributing to tTAF-driven gene expression. Decreased male fertility associated with mutation in one of the regulators, Acj6, indicates its involvement in male reproduction. Transcriptome study of testes from male mutants for tTAF, Acj6, and previously characterized tTAF-interacting factor Modulo implies the existence of a regulatory hierarchy of tTAF, Modulo and Acj6, in which Modulo and/or Acj6 regulate one-third of tTAF-dependent genes. </p>

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Author and article information

Journal

Title: FEBS Letters

Abbreviated Title: FEBS Lett

Publisher: Wiley

ISSN: 00145793

Publication date Created: January 2018

Publication date (Print): January 2018

Publication date (Electronic): December 27 2017

Volume: 592

Issue: 1

Pages: 46-59

Affiliations

[1 ]Shanghai Tenth People's Hospital; Tongji University School of Medicine; Shanghai China

[2 ]Department of Biochemistry and Molecular Biology; School of Medicine; University of Maryland; Baltimore MD USA

[3 ]Advanced Institute of Translational Medicine; Tongji University School of Medicine; Shanghai China

[4 ]Key Laboratory of Aquaculture Resources and Utilization; Ministry of Education; College of Fisheries and Life Science; Shanghai Ocean University; China