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      The effect of atmospheric pressure cold plasma on the inactivation of Escherichia coli in sour cherry juice and its qualitative properties

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          Abstract

          One of the nonthermal methods is the atmospheric pressure cold plasma (APCP). In this study, the effect of cold plasma on the reduction of Escherichia coli bacteria and qualitative properties of sour cherry juice, including total phenolic content (TPC), total anthocyanin content (TAC), and vitamin C, were investigated. Independent variables included plasma exposure time (1, 5, and 9 min), applied field intensity (25, 37.5, and 50 kV/cm), feeding gas oxygen content (0%, 0.5%, and 1%), and sample depth (0.5, 1, and 1.5 cm). The results show that increased oxygen content in argon has the greatest effect on the reduction of bacteria, and plasma exposure decreased 6 logarithmic periods of E. coli bacteria in sour cherry juice. Optimization results showed when all bacteria were eliminated by plasma, TPC remained unchanged, and TAC and vitamin C decreased by 4% and 21%, respectively, while thermal methods increased TPC by 23% and decreased TAC and vitamin C by 26% and 77%, respectively. These results indicate that, compared with conventional thermal methods, sour cherry juice pasteurization using APCP has little effect on the juice qualitative properties, and this method can serve as a suitable alternative to conventional thermal methods.

          Abstract

          These results indicate that, compared with conventional thermal methods, sour cherry juice pasteurization using atmospheric pressure cold plasma has little effect on the juice qualitative properties, and this method can serve as a suitable alternative to conventional thermal methods.

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          Plasma–liquid interactions: a review and roadmap

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            Physical Mechanisms of Inactivation of Bacillus subtilis Spores Using Cold Atmospheric Plasmas

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              Mechanisms of Inactivation by High-Voltage Atmospheric Cold Plasma Differ for Escherichia coli and Staphylococcus aureus.

              Atmospheric cold plasma (ACP) is a promising nonthermal technology effective against a wide range of pathogenic microorganisms. Reactive oxygen species (ROS) play a crucial inactivation role when air or other oxygen-containing gases are used. With strong oxidative stress, cells can be damaged by lipid peroxidation, enzyme inactivation, and DNA cleavage. Identification of ROS and an understanding of their role are important for advancing ACP applications for a range of complex microbiological issues. In this study, the inactivation efficacy of in-package high-voltage (80 kV [root mean square]) ACP (HVACP) and the role of intracellular ROS were investigated. Two mechanisms of inactivation were observed in which reactive species were found to either react primarily with the cell envelope or damage intracellular components. Escherichia coli was inactivated mainly by cell leakage and low-level DNA damage. Conversely, Staphylococcus aureus was mainly inactivated by intracellular damage, with significantly higher levels of intracellular ROS observed and little envelope damage. However, for both bacteria studied, increasing treatment time had a positive effect on the intracellular ROS levels generated.
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                Author and article information

                Contributors
                rostami.sajad@yahoo.com
                Journal
                Food Sci Nutr
                Food Sci Nutr
                10.1002/(ISSN)2048-7177
                FSN3
                Food Science & Nutrition
                John Wiley and Sons Inc. (Hoboken )
                2048-7177
                20 January 2020
                February 2020
                : 8
                : 2 ( doiID: 10.1002/fsn3.v8.2 )
                : 870-883
                Affiliations
                [ 1 ] Department of Mechanical Engineering of Biosystems Shahrekord University Shahrekord Iran
                [ 2 ] Medical Plants Research Center Basic Health Sciences Institute Shahrekord University of Medical Sciences Shahrekord Iran
                Author notes
                [*] [* ] Correspondence

                Sajad Rostami, Department of Mechanical Engineering of Biosystems, Shahrekord University, Shahrekord, Iran.

                Email: rostami.sajad@ 123456yahoo.com

                Author information
                https://orcid.org/0000-0002-0375-0558
                https://orcid.org/0000-0002-8563-7080
                https://orcid.org/0000-0003-0844-7571
                Article
                FSN31364
                10.1002/fsn3.1364
                7020306
                558b6ca2-60cf-41da-bd69-1927e7807a0b
                © 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.

                This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

                History
                : 23 August 2019
                : 14 September 2019
                : 21 September 2019
                Page count
                Figures: 7, Tables: 5, Pages: 14, Words: 10199
                Funding
                Funded by: Shahrekord University , open-funder-registry 10.13039/501100004883;
                Award ID: 96GRN1M1796
                Categories
                Original Research
                Original Research
                Custom metadata
                2.0
                February 2020
                Converter:WILEY_ML3GV2_TO_JATSPMC version:5.7.5 mode:remove_FC converted:14.02.2020

                cold plasma,escherichia coli,nonthermal method,response surface method,sour cherry juice

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