Muhammad Ashfaq:
ORCID: http://orcid.org/0000-0003-1351-4126
Role: ConceptualizationRole: Data curationRole: Formal analysisRole: Funding acquisitionRole:
InvestigationRole: MethodologyRole: Project administrationRole: ResourcesRole: SoftwareRole:
SupervisionRole: ValidationRole: VisualizationRole: Writing – original draft
Gergin Blagoev: Role: Data curationRole: Formal analysisRole: InvestigationRole: MethodologyRole:
Writing – original draft
Hafiz Muhammad Tahir: Role: Data curationRole: InvestigationRole: MethodologyRole: Resources
Arif M. Khan: Role: InvestigationRole: MethodologyRole: Resources
Muhammad Khalid Mukhtar: Role: Investigation
Saleem Akhtar: Role: Methodology
Abida Butt: Role: Investigation
Shahid Mansoor: Role: MethodologyRole: Resources
Paul D. N. Hebert: Role: ConceptualizationRole: Data curationRole: Funding acquisitionRole: MethodologyRole:
Project administrationRole: ResourcesRole: SupervisionRole: ValidationRole: VisualizationRole:
Writing – review & editing
Sebastian D. Fugmann: Role: Editor
Journal ID (nlm-ta): PLoS One
Journal ID (iso-abbrev): PLoS ONE
Journal ID (publisher-id): plos
Journal ID (pmc): plosone
Title:
PLoS ONE
Publisher:
Public Library of Science
(San Francisco, CA USA
)
ISSN
(Electronic):
1932-6203
Publication date
(Electronic):
22
May
2019
Publication date Collection: 2019
Volume: 14
Issue: 5
Electronic Location Identifier: e0217086
Affiliations
[1
]
Centre for Biodiversity Genomics, University of Guelph, Guelph, ON, Canada
[2
]
Department of Zoology, GC University, Lahore, Pakistan
[3
]
Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
[4
]
Department of Zoology, University of Sargodha, Sargodha, Pakistan
[5
]
Directorate of Entomology, Ayub Agricultural Research Institute, Faisalabad, Pakistan
[6
]
Department of Zoology, University of the Punjab, Lahore, Pakistan
[7
]
National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan
Chang Gung University, TAIWAN
Author notes
Competing Interests: The authors have declared that no competing interests exist.
Author information
Article
Publisher ID:
PONE-D-19-01391
DOI: 10.1371/journal.pone.0217086
PMC ID: 6530854
PubMed ID: 31116764
SO-VID: 5ae711ec-1b3d-4948-a831-469c76229030
Copyright © © 2019 Ashfaq et al
License:
This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited.
This study was enabled by grant 106106-001 “Engaging Developing Nations in iBOL” from
the International Development Research Centre in Canada and by grant HEC No. 20-1403/R&
D/09 “Sequencing DNA Barcodes of Economically Important Insect Species from Pakistan”
from the Higher Education Commission of Pakistan awarded to MA. Sequence analysis
was made possible by a grant from the Government of Canada through Genome Canada and
Ontario Genomics in support of the International Barcode of Life (iBOL) project awarded
to PDNH. This is a contribution to the Food From Thought project supported by the
Canada First Research Excellence Fund awarded to PDNH. The funders had no role in
study design, data collection and analysis, decision to publish, or preparation of
the manuscript.
Subject:
Research Article
Subject:
Biology and Life Sciences
Subject:
Organisms
Subject:
Eukaryota
Subject:
Animals
Subject:
Invertebrates
Subject:
Arthropoda
Subject:
Arachnida
Subject:
Spiders
Subject:
People and Places
Subject:
Geographical Locations
Subject:
Asia
Subject:
Pakistan
Subject:
Biology and life sciences
Subject:
Molecular biology
Subject:
Molecular biology techniques
Subject:
DNA barcoding
Subject:
Research and analysis methods
Subject:
Molecular biology techniques
Subject:
DNA barcoding
Subject:
Biology and life sciences
Subject:
Evolutionary biology
Subject:
Evolutionary systematics
Subject:
Molecular systematics
Subject:
DNA barcoding
Subject:
Biology and life sciences
Subject:
Taxonomy
Subject:
Evolutionary systematics
Subject:
Molecular systematics
Subject:
DNA barcoding
Subject:
Computer and information sciences
Subject:
Data management
Subject:
Taxonomy
Subject:
Evolutionary systematics
Subject:
Molecular systematics
Subject:
DNA barcoding
Subject:
Research and analysis methods
Subject:
Database and informatics methods
Subject:
Bioinformatics
Subject:
Sequence analysis
Subject:
DNA sequence analysis
Subject:
Biology and Life Sciences
Subject:
Evolutionary Biology
Subject:
Evolutionary Processes
Subject:
Speciation
Subject:
Cryptic Speciation
Subject:
Biology and life sciences
Subject:
Genetics
Subject:
DNA
Subject:
DNA libraries
Subject:
Biology and life sciences
Subject:
Biochemistry
Subject:
Nucleic acids
Subject:
DNA
Subject:
DNA libraries
Subject:
Biology and Life Sciences
Subject:
Evolutionary Biology
Subject:
Evolutionary Systematics
Subject:
Phylogenetics
Subject:
Phylogenetic Analysis
Subject:
Biology and Life Sciences
Subject:
Taxonomy
Subject:
Evolutionary Systematics
Subject:
Phylogenetics
Subject:
Phylogenetic Analysis
Subject:
Computer and Information Sciences
Subject:
Data Management
Subject:
Taxonomy
Subject:
Evolutionary Systematics
Subject:
Phylogenetics
Subject:
Phylogenetic Analysis
Subject:
Research and Analysis Methods
Subject:
Database and Informatics Methods
Subject:
Bioinformatics
Subject:
Sequence Analysis