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      Preliminary marker-based validation of a novel biplane fluoroscopy system

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      1 , 1 , 1 , 2 , 1 , 3 , 1 , 3 , 4 ,
      Journal of Foot and Ankle Research
      BioMed Central
      3rd Congress of the International Foot and Ankle Biomechanics Community
      11-13 March 2012

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          Abstract

          Background The use of biplane fluoroscopy to track bones in the foot is challenging, due to distortion, overlap and image artefact inherent in fluoroscopy systems and high speed photography. The accuracy and precision of these systems have been reported [1-4] and are presented here for our biplane fluoroscopy system. Materials and methods Biplane Fluoroscopy System: The system consists of two Philips BV Pulsera C-arms set in custom frames around a raised floor with a radiolucent imaging area. X-ray images are captured with high speed (1000fps) cameras. Validation Object: 1.6mm tantalum beads were placed in a machined block (wand) then measured to 7 microns with a Coordinate Measuring Machine to determine their centroid location. The wand was translated and rotated via a 1 micron precision stepper-motor for static validation, as well as manually swept through the field of view at ~0.5m/s for dynamic. Static Accuracy and Precision: accuracy was defined as the RMS error between the translation of the stepper-motor and the measured movement of the beads; precision is defined as the standard deviation of the bead locations. For rotation, accuracy was defined as the RMS error between the applied and measured rotation of the wand. Dynamic Accuracy and Precision: accuracy was defined as the RMS error between the known and measured inter-bead distance; precision was the standard deviation of the inter-bead distance. 3D location processing was accomplished using custom software written in MatLab to derive the 3D location of objects from two, time-synchronized, 2D fluoroscopy images of known spatial relationship. This software also compensates for the image distortion (Figure 1). Figure 1 Distortion plate before (left) and after (right) correction for pin-hole distortion and magnetic distortion. Results Translation: the overall RMS error was 0.066 mm, with a precision of ± 0.016 mm. Rotation: the RMS error was 0.125°. Dynamic motion: the overall RMS error was 0.126 mm, with a precision of ± 0.122 mm. Conclusions The accuracies and precision in the results are comparable to similar such systems in development to investigate other joints of the body [1-4]. We are currently developing and validating a marker-less technique for tracking the bones of the foot.

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          X-ray reconstruction of moving morphology (XROMM): precision, accuracy and applications in comparative biomechanics research.

          X-Ray Reconstruction of Moving Morphology (XROMM) comprises a set of 3D X-ray motion analysis techniques that merge motion data from in vivo X-ray videos with skeletal morphology data from bone scans into precise and accurate animations of 3D bones moving in 3D space. XROMM methods include: (1) manual alignment (registration) of bone models to video sequences, i.e., Scientific Rotoscoping; (2) computer vision-based autoregistration of bone models to biplanar X-ray videos; and (3) marker-based registration of bone models to biplanar X-ray videos. Here, we describe a novel set of X-ray hardware, software, and workflows for marker-based XROMM. Refurbished C-arm fluoroscopes retrofitted with high-speed video cameras offer a relatively inexpensive X-ray hardware solution for comparative biomechanics research. Precision for our biplanar C-arm hardware and analysis software, measured as the standard deviation of pairwise distances between 1 mm tantalum markers embedded in rigid objects, was found to be +/-0.046 mm under optimal conditions and +/-0.084 mm under actual in vivo recording conditions. Mean error in measurement of a known distance between two beads was within the 0.01 mm fabrication tolerance of the test object, and mean absolute error was 0.037 mm. Animating 3D bone models from sets of marker positions (XROMM animation) makes it possible to study skeletal kinematics in the context of detailed bone morphology. The biplanar fluoroscopy hardware and computational methods described here should make XROMM an accessible and useful addition to the available technologies for studying the form, function, and evolution of vertebrate animals.
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            Validation of a non-invasive fluoroscopic imaging technique for the measurement of dynamic knee joint motion.

            The accurate measurement of the in vivo knee joint kinematics in six degrees-of-freedom (6DOF) remains a challenge in biomedical engineering. We have adapted a dual fluoroscopic imaging system (DFIS) to investigate the various in vivo dynamic knee joint motions. This paper presents a thorough validation of the accuracy and repeatability of the DFIS system when used to measure 6DOF dynamic knee kinematics. First, the validation utilized standard geometric spheres made from different materials to demonstrate the capability of the DFIS technique to determine the object positions under changing speeds. The translational pose of the spheres could be recreated to less than 0.15+/-0.09 mm for velocities below 300 mm/s. Next, tantalum beads were inserted into the femur and tibia of two fresh frozen cadaver knees to compare the dynamic kinematics measured by matching knee models to the kinematics from the tantalum bead matching-a technique similar to Roentgen stereophotogrammetric analysis (RSA). Each cadaveric knee was attached to the crosshead of a tensile testing machine and vertically translated at a rate of 16.66 mm/s while images were captured with the DFIS. Subsequently, the tibia was held fixed and the femur manually flexed from full extension to 90 degrees of flexion, as the DFIS acquired images. In vitro translation of the cadaver knee using the tensile testing machine deviated from predicted values by 0.08+/-0.14 mm for the matched knee models. The difference between matching the knee and tantalum bead models during the dynamic flexion-extension motion of the knee was 0.1+/-0.65 degrees /s in flexion speed; 0.24+/-0.16 mm in posterior femoral translation; and 0.16+/-0.61 degrees in internal-external tibial rotation. Finally, we applied the method to investigate the knee kinematics of a living subject during a step ascent and treadmill gait. High repeatability was demonstrated for the in vivo application. Thus, the DFIS provides an easy and powerful tool for accurately determining 6DOF positions of the knee when performing daily functional activities.
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              X-ray reconstruction of moving morphology (XROMM): precision, accuracy and applications in comparative biomechanics research

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                Author and article information

                Conference
                J Foot Ankle Res
                J Foot Ankle Res
                Journal of Foot and Ankle Research
                BioMed Central
                1757-1146
                2012
                10 April 2012
                : 5
                : Suppl 1
                : O36
                Affiliations
                [1 ]VA RR&D Center of Excellence for Limb Loss Prevention and Prosthetic Engineering, Seattle, WA, 98108, USA
                [2 ]Departments of Radiology and Bioengineering, University of Washington, Seattle, WA, 98195-7117, USA
                [3 ]Department of Orthopaedics & Sports Medicine, University of Washington, Seattle, WA, 98195-6500, USA
                [4 ]Department of Mechanical Engineering, University of Washington, Seattle, WA, 98195, USA
                Article
                1757-1146-5-S1-O36
                10.1186/1757-1146-5-S1-O36
                3323657
                6d4c7ead-d883-4691-940a-3aca5c74d4bd
                Copyright ©2012 Iaquinto et al; licensee BioMed Central Ltd.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                3rd Congress of the International Foot and Ankle Biomechanics Community
                Sydney, Australia
                11-13 March 2012
                History
                Categories
                Oral Presentation

                Orthopedics
                Orthopedics

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