- Record: found
- Abstract: not found
- Article: not found
Cyclin/Cdk-Dependent Initiation of DNA Replication in a Human Cell-Free System
January 1997
January 1997
Read this article at
There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
We describe a cell-free system from HeLa cells that initiates DNA replication under
cell cycle control. G1 but not G2 phase nuclei initiate replication when coincubated
with S phase nuclei in cytosolic extracts from S phase but not from G1 or G2 phase
HeLa cells. S phase nuclei or an S phase nuclear extract are required for the initiation
of semiconservative DNA replication in G1 nuclei but not for elongation in S phase
nuclei. S phase nuclear extract could be replaced by recombinant human cyclins A and
E complexed to Cdk2 but not by Cdk2 alone or by human cyclin B1 complexed to Cdc2.
In S phase cytosol, cyclins A/Cdk2 and E/Cdk2 triggered initiation synergistically.