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      Draft Genome Sequence of Xanthomonas arboricola pv. juglandis J303, Isolated from Infected Walnut Trees in Southern Chile

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          ABSTRACT

          Here, we report the draft genome sequence of Xanthomonas arboricola pv. juglandis J303, isolated from infected walnut trees in southern Chile. The size of the genome is 5,066,424 bp with a G+C content of 65.4%. X. arboricola pv. juglandis J303 has several genes related to virulence, antibiotic resistance, and copper resistance.

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          Advances in Bacteriophage-Mediated Control of Plant Pathogens

          Rebekah Frampton, Andrew R. Pitman, Peter Fineran (2012)
          There is continuing pressure to maximise food production given a growing global human population. Bacterial pathogens that infect important agricultural plants (phytopathogens) can reduce plant growth and the subsequent crop yield. Currently, phytopathogens are controlled through management programmes, which can include the application of antibiotics and copper sprays. However, the emergence of resistant bacteria and the desire to reduce usage of toxic products that accumulate in the environment mean there is a need to develop alternative control agents. An attractive option is the use of specific bacteriophages (phages), viruses that specifically kill bacteria, providing a more targeted approach. Typically, phages that target the phytopathogen are isolated and characterised to determine that they have features required for biocontrol. In addition, suitable formulation and delivery to affected plants are necessary to ensure the phages survive in the environment and do not have a deleterious effect on the plant or target beneficial bacteria. Phages have been isolated for different phytopathogens and have been used successfully in a number of trials and commercially. In this paper, we address recent progress in phage-mediated control of plant pathogens and overcoming the challenges, including those posed by CRISPR/Cas and abortive infection resistance systems.
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            Improved silica-guanidiniumthiocyanate DNA isolation procedure based on selective binding of bovine alpha-casein to silica particles.

            R Boom, C. Sol, M. Beld … (1999)
            DNA purified from clinical cerebrospinal fluid and urine specimens by a silica-guanidiniumthiocyanate procedure frequently contained an inhibitor(s) of DNA-processing enzymes which may have been introduced by the purification procedure itself. Inhibition could be relieved by the use of a novel lysis buffer containing alpha-casein. When the novel lysis buffer was used, alpha-casein was bound by the silica particles in the first step of the procedure and eluted together with DNA in the last step, after which it exerted its beneficial effects for DNA-processing enzymes. In the present study we have compared the novel lysis buffer with the previously described lysis buffer with respect to double-stranded DNA yield (which was nearly 100%) and the performance of DNA-processing enzymes.
            Article 0 comments Cited 30 times – based on 0 reviews     Review now
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              Draft Genome Sequences of Four Xanthomonas arboricola pv. juglandis Strains Associated with Walnut Blight in Chile

              Gastón Higuera, Narjol Gonzalez-Escalona, Camila Véliz … (2015)
              Xanthomonas arboricola pv. juglandis is an important pathogen responsible for walnut blight outbreaks globally. Here, we report four draft genome sequences of X. arboricola pv. juglandis strains isolated from Chilean walnut trees.
              Article 0 comments Cited 15 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Genome Announc
                Journal ID (iso-abbrev): Genome Announc
                Journal ID (hwp): ga
                Journal ID (pmc): ga
                Journal ID (publisher-id): GA
                Title: Genome Announcements
                Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )
                ISSN (Electronic): 2169-8287
                Publication date (Electronic): 28 September 2017
                Publication date Collection: September 2017
                Volume: 5
                Issue: 39
                Electronic Location Identifier: e01085-17
                Affiliations
                [a ]Agroadvance Laboratory, Peñaflor, Chile
                [b ]Instituto de Ciencias Naturales, Universidad de Las Americas, Viña del Mar, Chile
                [c ]Faculty of Sciences, Universidad Mayor, Huechuraba, Chile
                [d ]Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, Canada
                Author notes
                Address correspondence to Julio Retamales, jretamales@ 123456agroadvance.cl , or Javier Santander, jsantander@ 123456mun.ca .
                Author information
                http://orcid.org/0000-0002-7701-3147
                Article
                Publisher ID: genomeA01085-17
                DOI: 10.1128/genomeA.01085-17
                PMC ID: 5624772
                PubMed ID: 28963226
                SO-VID: aa0738b2-cc0c-4036-81bb-f0b2d12f7e1f
                Copyright © Copyright © 2017 Retamales et al.
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

                History
                Date received : 30 August 2017
                Date accepted : 1 September 2017
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 14, Pages: 2, Words: 1168
                Funding
                Funded by: MINEDUC | Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) https://doi.org/10.13039/501100002848
                Award ID: 1140330
                Award Recipient : Javier Santander
                Categories
                Subject: Prokaryotes
                Custom metadata
                cover-date September 2017

                ScienceOpen disciplines: Genetics
                Data availability:
                ScienceOpen disciplines: Genetics

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