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      Bringing cultured meat to market: Technical, socio-political, and regulatory challenges in cellular agriculture

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          Abstract

          Background

          Cultured meat forms part of the emerging field of cellular agriculture. Still an early stage field it seeks to deliver products traditionally made through livestock rearing in novel forms that require no, or significantly reduced, animal involvement. Key examples include cultured meat, milk, egg white and leather. Here, we focus upon cultured meat and its technical, socio-political and regulatory challenges and opportunities.

          Scope and approach

          The paper reports the thinking of an interdisciplinary team, all of whom have been active in the field for a number of years. It draws heavily upon the published literature, as well as our own professional experience. This includes ongoing laboratory work to produce cultured meat and over seventy interviews with experts in the area conducted in the social science work.

          Key findings and conclusions

          Cultured meat is a promising, but early stage, technology with key technical challenges including cell source, culture media, mimicking the in-vivo myogenesis environment, animal-derived and synthetic materials, and bioprocessing for commercial-scale production. Analysis of the social context has too readily been reduced to ethics and consumer acceptance, and whilst these are key issues, the importance of the political and institutional forms a cultured meat industry might take must also be recognised, and how ambiguities shape any emergent regulatory system.

          Highlights

          • Cellular agriculture includes tissue engineering and fermentation based approaches.

          • Five key technical challenges with cultured meat production are made explicit.

          • Social issue studies disproportionately focus upon ethics and consumer acceptance.

          • New analysis of political, institutional and regulatory issues is required.

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          Most cited references76

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          Three-dimensional bioprinting of thick vascularized tissues.

          The advancement of tissue and, ultimately, organ engineering requires the ability to pattern human tissues composed of cells, extracellular matrix, and vasculature with controlled microenvironments that can be sustained over prolonged time periods. To date, bioprinting methods have yielded thin tissues that only survive for short durations. To improve their physiological relevance, we report a method for bioprinting 3D cell-laden, vascularized tissues that exceed 1 cm in thickness and can be perfused on chip for long time periods (>6 wk). Specifically, we integrate parenchyma, stroma, and endothelium into a single thick tissue by coprinting multiple inks composed of human mesenchymal stem cells (hMSCs) and human neonatal dermal fibroblasts (hNDFs) within a customized extracellular matrix alongside embedded vasculature, which is subsequently lined with human umbilical vein endothelial cells (HUVECs). These thick vascularized tissues are actively perfused with growth factors to differentiate hMSCs toward an osteogenic lineage in situ. This longitudinal study of emergent biological phenomena in complex microenvironments represents a foundational step in human tissue generation.
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            Scaffolding in tissue engineering: general approaches and tissue-specific considerations.

            B Chan, K Leong (2008)
            Scaffolds represent important components for tissue engineering. However, researchers often encounter an enormous variety of choices when selecting scaffolds for tissue engineering. This paper aims to review the functions of scaffolds and the major scaffolding approaches as important guidelines for selecting scaffolds and discuss the tissue-specific considerations for scaffolding, using intervertebral disc as an example.
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              The skeletal muscle satellite cell: the stem cell that came in from the cold.

              The muscle satellite cell was first described and actually named on the basis of its anatomic location under the basement membrane surrounding each myofiber. For many years following its discovery, electron microscopy provided the only definitive method of identification. More recently, several molecular markers have been described that can be used to detect satellite cells, making them more accessible for study at the light microscope level. Satellite cells supply myonuclei to growing myofibers before becoming mitotically quiescent in muscle as it matures. They are then activated from this quiescent state to fulfill their roles in routine maintenance, hypertrophy, and repair of adult muscle. Because muscle is able to efficiently regenerate after repeated bouts of damage, systems must be in place to maintain a viable satellite cell pool, and it was proposed over 30 years ago that self-renewal was the primary mechanism. Self-renewal entails either a stochastic event or an asymmetrical cell division, where one daughter cell is committed to differentiation whereas the second continues to proliferate or becomes quiescent. This classic model of satellite cell self-renewal and the importance of satellite cells in muscle maintenance and repair have been challenged during the past few years as bone marrow-derived cells and various intramuscular populations were shown to be able to contribute myonuclei and occupy the satellite cell niche. This is a fast-moving and dynamic field, however, and in this review we discuss the evidence that we think puts this enigmatic cell firmly back at the center of adult myogenesis.
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                Author and article information

                Contributors
                Journal
                Trends Food Sci Technol
                Trends Food Sci Technol
                Trends in Food Science & Technology
                Elsevier Trends Journals
                0924-2244
                1879-3053
                1 August 2018
                August 2018
                : 78
                : 155-166
                Affiliations
                [a ]Social and Political Sciences, Brunel University London, Kingston Lane, Uxbridge, UB8 3PH, United Kingdom
                [b ]Charcutier Ltd, Felin y Glyn Farm, Pontnewydd, Llanelli, SA15 5TL, United Kingdom
                [c ]Kings College London, Floor 17, Tower Wing Guy's London, United Kingdom
                [d ]Dept of Chemical Engineering, Claverton Down, Bath, BA2 7AY, United Kingdom
                [e ]Weston Park Farm, Weston, SG1 7BX, United Kingdom
                [f ]Oxford Martin School, University of Oxford, 34 Broad Street, Oxford, OX1 3BD, United Kingdom
                Author notes
                []Corresponding author. neil.stephens@ 123456brunel.ac.uk
                [1]

                All authors contributed equally.

                Article
                S0924-2244(17)30340-0
                10.1016/j.tifs.2018.04.010
                6078906
                30100674
                b6484147-ccb0-4a1c-a6b9-1365739095e7
                © 2018 The Authors

                This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

                History
                : 2 June 2017
                : 24 April 2018
                : 25 April 2018
                Categories
                Article

                cellular agriculture,cultured meat,clean meat,in vitro meat,society,regulation

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