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      Protein profile of rice ( Oryza sativa) seeds

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          Abstract

          Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice ( Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4–7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic and were related to binding, catalytic, cellular or metabolic processes. These results expand our knowledge of the rice proteome and improve our understanding of the cellular biology of rice seeds.

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          Seed dormancy and the control of germination.

          William E Finch-Savage, Gerhard Leubner-Metzger (2006)
          Seed dormancy is an innate seed property that defines the environmental conditions in which the seed is able to germinate. It is determined by genetics with a substantial environmental influence which is mediated, at least in part, by the plant hormones abscisic acid and gibberellins. Not only is the dormancy status influenced by the seed maturation environment, it is also continuously changing with time following shedding in a manner determined by the ambient environment. As dormancy is present throughout the higher plants in all major climatic regions, adaptation has resulted in divergent responses to the environment. Through this adaptation, germination is timed to avoid unfavourable weather for subsequent plant establishment and reproductive growth. In this review, we present an integrated view of the evolution, molecular genetics, physiology, biochemistry, ecology and modelling of seed dormancy mechanisms and their control of germination. We argue that adaptation has taken place on a theme rather than via fundamentally different paths and identify similarities underlying the extensive diversity in the dormancy response to the environment that controls germination.
          Article 0 comments Cited 628 times – based on 0 reviews     Review now
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            Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana.

            M Bevan, I Bancroft, E. Bent … (1998)
            The plant Arabidopsis thaliana (Arabidopsis) has become an important model species for the study of many aspects of plant biology. The relatively small size of the nuclear genome and the availability of extensive physical maps of the five chromosomes provide a feasible basis for initiating sequencing of the five chromosomes. The YAC (yeast artificial chromosome)-based physical map of chromosome 4 was used to construct a sequence-ready map of cosmid and BAC (bacterial artificial chromosome) clones covering a 1.9-megabase (Mb) contiguous region, and the sequence of this region is reported here. Analysis of the sequence revealed an average gene density of one gene every 4.8 kilobases (kb), and 54% of the predicted genes had significant similarity to known genes. Other interesting features were found, such as the sequence of a disease-resistance gene locus, the distribution of retroelements, the frequent occurrence of clustered gene families, and the sequence of several classes of genes not previously encountered in plants.
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              Proteomic analysis of rice (Oryza sativa) seeds during germination.

              Xiaoqin Wang, Shihua Shen, Fan Chen … (2007)
              Although seed germination is a major subject in plant physiological research, there is still a long way to go to elucidate the mechanism of seed germination. Recently, functional genomic strategies have been applied to study the germination of plant seeds. Here, we conducted a proteomic analysis of seed germination in rice (Oryza sativa indica cv. 9311) - a model monocot. Comparison of 2-DE maps showed that there were 148 proteins displayed differently in the germination process of rice seeds. Among the changed proteins, 63 were down-regulated, 69 were up-regulated (including 20 induced proteins). The down-regulated proteins were mainly storage proteins, such as globulin and glutelin, and proteins associated with seed maturation, such as "early embryogenesis protein" and "late embryogenesis abundant protein", and proteins related to desiccation, such as "abscisic acid-induced protein" and "cold-regulated protein". The degradation of storage proteins mainly happened at the late stage of germination phase II (48 h imbibition), while that of seed maturation and desiccation associated proteins occurred at the early stage of phase II (24 h imbibition). In addition to alpha-amylase, the up-regulated proteins were mainly those involved in glycolysis such as UDP-glucose dehydrogenase, fructokinase, phosphoglucomutase, and pyruvate decarboxylase. The results reflected the possible biochemical and physiological processes of germination of rice seeds.
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                Author and article information

                Journal
                Journal ID (nlm-ta): Genet Mol Biol
                Journal ID (iso-abbrev): Genet. Mol. Biol
                Journal ID (publisher-id): GMB
                Title: Genetics and Molecular Biology
                Publisher: Sociedade Brasileira de Genética (Ribeirão Preto, SP, Brazil )
                ISSN (Print): 1415-4757
                ISSN (Electronic): 1678-4685
                Publication date (Electronic): 4 March 2013
                Publication date (Print): March 2013
                Volume: 36
                Issue: 1
                Pages: 87-92
                Affiliations
                Institute of Life Sciences, Jiangsu University, Zhenjiang, PR China.
                Author notes
                Send correspondence to Keping Chen. Institute of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu Province 212013, PR China. E-mail: kpchen@ 123456ujs.edu.cn .
                [#]

                These authors contributed equally to this work.

                Article
                Publisher ID: gmb-36-87
                DOI: 10.1590/S1415-47572013000100012
                PMC ID: 3615530
                PubMed ID: 23569412
                SO-VID: ba5a0dc4-2251-4769-ad39-9ab18a4377ae
                Copyright © Copyright © 2013, Sociedade Brasileira de Genética.
                License:

                License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 06 July 2012
                Date accepted : 23 October 2012
                Categories
                Subject: Plant Genetics
                Subject: Research Article

                ScienceOpen disciplines: Molecular biology
                Keywords: mass spectrometry,proteomic analysis,rice seed,two-dimensional electrophoresis
                Data availability:
                ScienceOpen disciplines: Molecular biology
                Keywords: mass spectrometry, proteomic analysis, rice seed, two-dimensional electrophoresis

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