A biochemical, theoretical and immunohistochemical study comparing the therapeutic efficacy of curcumin and taurine on T-2 toxin induced hepatotoxicity in rats

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Abstract

Introduction: Foodborne trichothecene T-2 Toxin, is a highly toxic metabolite produced by Fusarium species contaminating animal and human food, causing multiple organ failure and health hazards. T-2 toxins induce hepatotoxicity via oxidative stress causing hepatocytes cytotoxicity and genotoxicity. In this study, curcumin and taurine were investigated and compared as antioxidants against T-2-provoked hepatotoxicity.

Methods: Wistar rats were administrated T-2 toxin sublethal oral dose (0.1 mg/kg) for 2 months, followed by curcumin (80 mg/kg) and taurine (50 mg/kg) for 3 weeks. Biochemical assessment of liver enzymes, lipid profiles, thiobarbituric acid reactive substances (TBARs), AFU, TNF-α, total glutathione, molecular docking, histological and immunohistochemical markers for anti-transforming growth factor-β1 (TGFβ1), double-strand DNA damage (H2AX), regeneration (KI67) and apoptosis (Active caspase3) were done.

Results and Discussion: Compared to T-2 toxin, curcumin and taurine treatment significantly ameliorated hepatoxicity as; hemoglobin, hematocrit and glutathione, hepatic glycogen, and KI-67 immune-reactive hepatocytes were significantly increased. Although, liver enzymes, inflammation, fibrosis, TGFβ1 immunoexpressing and H2AX and active caspase 3 positive hepatocytes were significantly decreased. Noteworthy, curcumin’s therapeutic effect was superior to taurine by histomorphometry parameters. Furthermore, molecular docking of the structural influence of curcumin and taurine on the DNA sequence showed curcumin’s higher binding affinity than taurine.

Conclusion: Both curcumin and taurine ameliorated T-2 induced hepatotoxicity as strong antioxidative agents with more effectiveness for curcumin.

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Most cited references 101

Record: found
Abstract: found
Article: not found

UCSF Chimera--a visualization system for exploratory research and analysis.

Eric F. Pettersen, Thomas Goddard, Conrad Huang … (2004)

The design, implementation, and capabilities of an extensible visualization system, UCSF Chimera, are discussed. Chimera is segmented into a core that provides basic services and visualization, and extensions that provide most higher level functionality. This architecture ensures that the extension mechanism satisfies the demands of outside developers who wish to incorporate new features. Two unusual extensions are presented: Multiscale, which adds the ability to visualize large-scale molecular assemblies such as viral coats, and Collaboratory, which allows researchers to share a Chimera session interactively despite being at separate locales. Other extensions include Multalign Viewer, for showing multiple sequence alignments and associated structures; ViewDock, for screening docked ligand orientations; Movie, for replaying molecular dynamics trajectories; and Volume Viewer, for display and analysis of volumetric data. A discussion of the usage of Chimera in real-world situations is given, along with anticipated future directions. Chimera includes full user documentation, is free to academic and nonprofit users, and is available for Microsoft Windows, Linux, Apple Mac OS X, SGI IRIX, and HP Tru64 Unix from http://www.cgl.ucsf.edu/chimera/. Copyright 2004 Wiley Periodicals, Inc.

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Record: found
Abstract: found
Article: not found

ROS function in redox signaling and oxidative stress.

Michael Schieber, Navdeep S. Chandel (2014)

Oxidative stress refers to elevated intracellular levels of reactive oxygen species (ROS) that cause damage to lipids, proteins and DNA. Oxidative stress has been linked to a myriad of pathologies. However, elevated ROS also act as signaling molecules in the maintenance of physiological functions--a process termed redox biology. In this review we discuss the two faces of ROS--redox biology and oxidative stress--and their contribution to both physiological and pathological conditions. Redox biology involves a small increase in ROS levels that activates signaling pathways to initiate biological processes, while oxidative stress denotes high levels of ROS that result in damage to DNA, protein or lipids. Thus, the response to ROS displays hormesis, given that the opposite effect is observed at low levels compared with that seen at high levels. Here, we argue that redox biology, rather than oxidative stress, underlies physiological and pathological conditions. Copyright © 2014 Elsevier Ltd. All rights reserved.

0 comments Cited 1217 times – based on 0 reviews      Review now

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Record: found
Abstract: found
Article: not found

Bioavailability of curcumin: problems and promises.

Preetha Anand, Ajaikumar Kunnumakkara, Robert A. Newman … (2007)

Curcumin, a polyphenolic compound derived from dietary spice turmeric, possesses diverse pharmacologic effects including anti-inflammatory, antioxidant, antiproliferative and antiangiogenic activities. Phase I clinical trials have shown that curcumin is safe even at high doses (12 g/day) in humans but exhibit poor bioavailability. Major reasons contributing to the low plasma and tissue levels of curcumin appear to be due to poor absorption, rapid metabolism, and rapid systemic elimination. To improve the bioavailability of curcumin, numerous approaches have been undertaken. These approaches involve, first, the use of adjuvant like piperine that interferes with glucuronidation; second, the use of liposomal curcumin; third, curcumin nanoparticles; fourth, the use of curcumin phospholipid complex; and fifth, the use of structural analogues of curcumin (e.g., EF-24). The latter has been reported to have a rapid absorption with a peak plasma half-life. Despite the lower bioavailability, therapeutic efficacy of curcumin against various human diseases, including cancer, cardiovascular diseases, diabetes, arthritis, neurological diseases and Crohn's disease, has been documented. Enhanced bioavailability of curcumin in the near future is likely to bring this promising natural product to the forefront of therapeutic agents for treatment of human disease.

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Author and article information

Contributors

Maryam H. Al-Zahrani: URI : https://loop.frontiersin.org/people/1181221/overview

Maha J. Balgoon: URI : https://loop.frontiersin.org/people/1308607/overview

Lina A. Baz: URI : https://loop.frontiersin.org/people/1978717/overview

Sahar M. Gebril: URI : https://loop.frontiersin.org/people/2285765/overview

Journal

Journal ID (nlm-ta): Front Mol Biosci

Journal ID (iso-abbrev): Front Mol Biosci

Journal ID (publisher-id): Front. Mol. Biosci.

Title: Frontiers in Molecular Biosciences

Publisher: Frontiers Media S.A.

ISSN (Electronic): 2296-889X

Publication date (Electronic): 04 May 2023

Publication date Collection: 2023

Volume: 10

Electronic Location Identifier: 1172403

Affiliations

[1] ¹ Biochemistry Department , Faculty of Science , King Abdulaziz University , Jeddah, Saudi Arabia

[2] ² Department of Chemistry , Faculty of Science , Sohag University , Sohag, Egypt

[3] ³ Ibn Sina National College for Medical Studies , Jeddah, Saudi Arabia

[4] ⁴ Biochemistry Department , Faculty of Pharmacy , Beni Suef University , Beni Suef, Egypt

[5] ⁵ Histology and Cell biology Department , Faculty of Medicine , Sohag University , Sohag, Egypt

Author notes

Edited by: Enis Kostallari, Mayo Clinic, United States

Reviewed by: Yiling Li, The First Affiliated Hospital of China Medical University, China

Jasim Khan, University of Alabama at Birmingham, United States

*Correspondence: Maryam H. Al-Zahrani, mhsalzahrani@ 123456kau.edu.sa

[ † ]

ORCID: Maryam H. Al-Zahrani, orcid.org/0000-0002-1234-7947; Sahar M Gebril, orcid.org/0000-0002-8679-4897

Article

Publisher ID: 1172403

DOI: 10.3389/fmolb.2023.1172403

PMC ID: 10192634

PubMed ID: 37214337

SO-VID: cc51a2d0-56c8-4e2c-92c8-2e66446c61d2

License:

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