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      XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos.

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          Abstract

          XTcf-3 is a maternally expressed Xenopus homolog of the mammalian HMG box factors Tcf-1 and Lef-1. The N-terminus of XTcf-3 binds to beta-catenin. Microinjection of XTcf-3 mRNA in embryos results in nuclear translocation of beta-catenin. The beta-catenin-XTcf-3 complex activates transcription in a transient reporter gene assay, while XTcf-3 by itself is silent. N-terminal deletion of XTcf-3 (delta N) abrogates the interaction with beta-catenin, as well as the consequent transcription activation. This dominant-negative delta N mutant suppresses the induction of axis duplication by microinjected beta-catenin. It also suppresses endogenous axis specification upon injection into the dorsal blastomeres of a 4-cell-stage embryo. We propose that signaling by beta-catenin involves complex formation with XTcf-3, followed by nuclear translocation and activation of specific XTcf-3 target genes.

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          Author and article information

          Journal
          Cell
          Cell
          Elsevier BV
          0092-8674
          0092-8674
          Aug 09 1996
          : 86
          : 3
          Affiliations
          [1 ] Department of Immunology University Hospital, Utrecht The Netherlands.
          Article
          S0092-8674(00)80112-9
          10.1016/s0092-8674(00)80112-9
          8756721
          da34dc9f-5008-41a7-a614-43c801c8408b

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