+1 Recommend
0 collections
      • Record: found
      • Abstract: found
      • Article: not found

      Cloning of a rat gene encoding the histo-blood group B enzyme: rats have more than one Abo gene.


      ABO Blood-Group System, genetics, Amino Acid Sequence, Animals, CHO Cells, COS Cells, Cloning, Molecular, Cricetinae, DNA, Complementary, metabolism, Exons, Galactosyltransferases, Humans, Introns, Mice, Molecular Sequence Data, N-Acetylgalactosaminyltransferases, RNA, Messenger, biosynthesis, Rats, Rats, Inbred Strains, Rats, Sprague-Dawley, Rats, Wistar, Sequence Analysis, DNA, Sequence Homology, Amino Acid

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          A genomic DNA fragment corresponding to exon 7 of the human ABO gene was amplified from rats of several inbred and outbred strains. Five different sequences were obtained, four of them corresponding to A-type sequences and one to a B-type sequence based on the amino acids equivalent to residues at positions 266 and 268 of the human enzymes. In rats from inbred strains, a single A-type sequence and the unique B-type sequence were found, whereas some animals of outbred strains presented two or three A-type sequences along with the B-type sequence. The complete coding sequence of the B-type gene was obtained; identification of the exon-intron boundaries, determined by comparison with rat genomic sequences from data banks, revealed that the rat B-type gene structure is identical with that of the mouse Abo gene. Compared with the human ABO gene and the rat A gene, it lacks exon 4. Like the rat A gene (symbol: Abo), the rat B gene (symbol: Abo2) is located on chromosome 3q11-q12. It could be shown by transfection experiments that the B-type cDNA encodes an active B transferase. A transcript of the B gene was found ubiquitously, whereas the B antigen was only detected in a restricted set of tissues. These data indicate that rats have at least two distinct Abo genes, one monomorphic gene encoding a B-specific enzyme and one or more genes in some cases encoding an A-specific enzyme.

          Related collections

          Author and article information



          Comment on this article