Draft Genome Sequence of Alkane-Degrading Acinetobacter venetianus JKSF02, Isolated from Contaminated Sediment of the San Jacinto River in Houston, Texas

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Abstract

Acinetobacter venetianus JKSF02 was isolated from contaminated sediment in eastern Houston, Texas along the San Jacinto River. This microorganism specializes in n-alkane degradation and is well suited for bioremediation of the petroleum hydrocarbon deposited throughout the region by shipping and industrial activity from the Houston Ship Channel.

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Detection and location of OP-degrading activity: A model to integrate education and research.

Rupa Iyer, Kevin Smith, Bill Kudrle … (2015)

The Environmental Sampling Research Module (ESRM) is an investigative/discovery module that provides undergraduate research experiences for students as part of an interdisciplinary research-based biotechnology curriculum at the University of Houston campus. As part of the ESRM, students collect soil samples from various locations to test for the presence of organophosphorous (OP) degrading bacteria. At the end of this research project students submit a research paper on their field and laboratory activities and discuss their experimental data and observations. Students also record the date, location of collection, and the results of testing the sample for the degradation of two pesticides, methyl parathion or paraoxon, in an electronic laboratory notebook (ELN). Each collection site is recorded on a Google Maps module and the data from student research activities is made available to other undergraduate students. This data is then used to generate a microorganism database of pesticide degrading activity and promote reading, critical thinking, and analytical skills as part of the curriculum. Our sampling of agricultural sites and wastewater within and around the city of Houston has identified seven distinct genera of OP degrading organisms, including Pseudomonas, Stenotrophomonas, Exiguobacterium, Delftia, Agrobacterium, Aeromonas, and Rhizobium. Collected strains exhibit phosphotriesterase-like enzymatic activity with isolates of Pseudomonas putida and Stenotrophomonas maltophilia capable of degrading both the phosphotriester paraoxon and the phosphorothioate methyl parathion. Using this collection of OP-degrading microorganisms, undergraduate students have evaluated their potential for enhancing the removal of harmful organophosphates and their toxic metabolites from contaminated agricultural soil and adjacent bodies of water. This analytical data can potentially be utilized for environmental and industrial applications in bioremediation and ecology providing an innovative method for integrating education and research. In addition, the versatility of the ESRM itself provides for easy and rapid adaptation into varying environmental science courses with significant potential for the discovery and isolation of new and unique organisms to be used as part of ongoing research in the laboratory.

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Draft genome sequence of the hydrocarbon-degrading and emulsan-producing strain Acinetobacter venetianus RAG-1T.

Renato Fani, Giovanni Emiliani, Lenie Dijkshoorn … (2012)

We report the draft genome sequence of Acinetobacter venetianus strain RAG-1(T), which is able to degrade hydrocarbons and to synthesize a powerful biosurfactant (emulsan) that can be employed for oil removal and as an adjuvant for vaccine delivery. The genome sequence of A. venetianus RAG-1(T) might be useful for bioremediation and/or clinical purposes.

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Distribution of polychlorinated dibenzo-p-dioxins and dibenzofurans in suspended sediments, dissolved phase and bottom sediment in the Houston Ship Channel.

Monica Suarez, Hanadi Rifai, Randy M. Palachek … (2006)

Spatial distributions of polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) in the water column and bottom sediments of the Houston Ship Channel in Texas were measured three times over a 1-year period. Total TEQ concentrations in water ranged from 0.01 to 0.25 pg/l for the dissolved phase and from 0.09 to 2.91 pg/l for the suspended phase, while TEQ concentrations in bottom sediments varied from 0.9 to 139.8 ng/kg dry wt. The dissolved concentrations were lower than their respective suspended concentrations, with average dissolved/suspended ratios between 0.11 and 0.59 for individual congeners. More than 89% of the total concentration of 2378-substituted PCDD/PCDFs was attributable to OCDD but 2378-TCDD was the major contributor to total TEQ for the three sampled media. Average logs of organic carbon-normalized suspended sediment-dissolved partitioning coefficients (logK(oc)(obs)) varied between 4.92 and 8.59 l/kg-oc; while in the bottom sediment-dissolved interface, logK(oc)(obs) values ranged from 5.48 to 8.48 l/kg-oc. Observed logK(oc)values varied within a factor of 0.64-1.26 from equilibrium logK(oc) values, suggesting fluxes of PCDD/PCDFs across the interfaces. It was found that in the HSC, on average, the tendency of a compound to move from the particulate phase to the dissolved phase decreases with increasing K(ow).

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Author and article information

Journal

Journal ID (nlm-ta): Genome Announc

Journal ID (iso-abbrev): Genome Announc

Journal ID (hwp): ga

Journal ID (pmc): ga

Journal ID (publisher-id): GA

Title: Genome Announcements

Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )

ISSN (Electronic): 2169-8287

Publication date (Electronic): 14 April 2016

Publication date Collection: Mar-Apr 2016

Volume: 4

Issue: 2

Electronic Location Identifier: e00286-16

Affiliations

[a ]Center for Life Sciences Technology, College of Technology, University of Houston, Houston, Texas, USA

[b ]Department of Pediatrics, Section of Pediatric Tropical Medicine, Baylor College of Medicine, Houston, Texas, USA

Author notes

Address correspondence to Rupa Iyer, riyer@ 123456uh.edu .

Article

Publisher ID: genomeA00286-16

DOI: 10.1128/genomeA.00286-16

PMC ID: 4832172

PubMed ID: 27081144

SO-VID: 1202128d-52c0-42ca-b73a-5fd637d5f639

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

History

Date received : 29 February 2016

Date accepted : 2 March 2016

Page count

Figures: 0, Tables: 0, Equations: 0, References: 7, Pages: 2, Words: 946

Funding

Funding for this work was provided by the National Science Foundation (award no. 1505403). The NSF had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

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cover-date March/April 2016

ScienceOpen disciplines: Genetics

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ScienceOpen disciplines: Genetics

Draft Genome Sequence of Alkane-Degrading Acinetobacter venetianus JKSF02, Isolated from Contaminated Sediment of the San Jacinto River in Houston, Texas

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Most cited references 3

Detection and location of OP-degrading activity: A model to integrate education and research.

Draft genome sequence of the hydrocarbon-degrading and emulsan-producing strain Acinetobacter venetianus RAG-1T.

Distribution of polychlorinated dibenzo-p-dioxins and dibenzofurans in suspended sediments, dissolved phase and bottom sediment in the Houston Ship Channel.

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