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      Genome Sequence of Lactococcus lactis subsp. lactis NCDO 2118, a GABA-Producing Strain

      brief-report
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      Genome Announcements
      American Society for Microbiology

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          Abstract

          Lactococcus lactis subsp. lactis NCDO 2118 is a nondairy lactic acid bacterium, a xylose fermenter, and a gamma-aminobutyric acid (GABA) producer isolated from frozen peas. Here, we report the complete genome sequence of L. lactis NCDO 2118, a strain with probiotic potential activity.

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          Artemis: sequence visualization and annotation

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            CONTIGuator: a bacterial genomes finishing tool for structural insights on draft genomes

            Recent developments in sequencing technologies have given the opportunity to sequence many bacterial genomes with limited cost and labor, compared to previous techniques. However, a limiting step of genome sequencing is the finishing process, needed to infer the relative position of each contig and close sequencing gaps. An additional degree of complexity is given by bacterial species harboring more than one replicon, which are not contemplated by the currently available programs. The availability of a large number of bacterial genomes allows geneticists to use complete genomes (possibly from the same species) as templates for contigs mapping. Here we present CONTIGuator, a software tool for contigs mapping over a reference genome which allows the visualization of a map of contigs, underlining loss and/or gain of genetic elements and permitting to finish multipartite genomes. The functionality of CONTIGuator was tested using four genomes, demonstrating its improved performances compared to currently available programs. Our approach appears efficient, with a clear visualization, allowing the user to perform comparative structural genomics analysis on draft genomes. CONTIGuator is a Python script for Linux environments and can be used on normal desktop machines and can be downloaded from http://contiguator.sourceforge.net.
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              The lactic acid bacteria: a literature survey.

              The purpose of this review article on the lactic acid bacteria grew from an early curiosity and a desire to convey and impart the broad scope of literary information on their functions as starter cultures, in the manufacture of fermentation products such as dairy products and alcoholic beverages, as well as their contribution to better health. This review article is an attempt to empower the reader and to circumvent the difficult task in acquiring and elucidating a large body of information. The intent is to familiarize the reader with the various lactic species, their habitat or source, associated food, physiological characteristics, colonial morphology, biochemical characteristics, culture media (enrichment, nonselective, and selective), classic description, and taxonomy. This review provides information on Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Carnobacterium, and Enterococcus. Trends are presented, such as the use of nisin to extend food shelf-life and the current research premise that Probiotic strains may alter the intestinal flora and thus prevent intestinal wall penetration by pathogens.
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                Author and article information

                Journal
                Genome Announc
                Genome Announc
                ga
                ga
                GA
                Genome Announcements
                American Society for Microbiology (1752 N St., N.W., Washington, DC )
                2169-8287
                2 October 2014
                Sep-Oct 2014
                : 2
                : 5
                : e00980-14
                Affiliations
                [a ]Laboratory of Cellular and Molecular Genetics, Institute of Biologic Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
                [b ]Institute of Biologic Sciences, Federal University of Pará, Belém, PA, Brazil
                [c ]AQUACEN, National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
                Author notes
                Address correspondence to Vasco Azevedo, vasco@ 123456icb.ufmg.br .
                [*]

                Present address: Siomar C. Soares, AQUACEN, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil; Anderson R. Santos, Federal University of Uberlândia, Minas Gerais, MG, Brazil; Ulisses P. Pereira, Federal University of Uberlândia, Minas Gerais, MG, Brazil.

                Article
                genomeA00980-14
                10.1128/genomeA.00980-14
                4183873
                25278529
                9db3420f-fd9f-43ff-b61e-86b3ea1b4993
                Copyright © 2014 Oliveira et al.

                This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

                History
                : 21 August 2014
                : 26 August 2014
                Page count
                Pages: 2
                Categories
                Prokaryotes
                Custom metadata
                September/October 2014
                free

                Genetics
                Genetics

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