An unusual clonal cytogenetic abnormality with t(15;17)(p11;q21) in a patient with severe aplastic anemia

Abstract

Sir, Aplastic anemia is a rare, serious disease characterized by pancytopenia and hypocellular bone marrow. We present a case of severe aplastic anemia with a novel cytogenetic abnormality involving a balanced translocation between chromosomes 15p11 and 17q21. The breakpoint in chromosome 17q21 was similar to that involved in acute promyelocytic leukemia. A 68-year-old male presented with complaints of progressively increasing weakness and low-grade fever for 30 days. There was no bleeding from any site. Past medical history and family history were non-contributory. Clinical evaluation revealed palor with no lymphadenopathy or organomegaly. Hematological workup revealed pancytopenia with hemoglobin 6.8 g/dl, total leukocyte count 1.2 × 109/l, absolute neutrophil count 0.24 × 109/l, platelet count 26 × 109/l, and peripheral blood differential count with neutrophils 20% and lymphocytes 80%. Bone marrow biopsy revealed hypocellular marrow with overall cellularity less than 5%. There was no marrow dysplasia, tumor infiltration, or myelofibrosis. Investigations for viral infections like hepatitis B virus, hepatitis C virus, human immunodeficiency virus, and parvovirus B19 were negative. Chromosomal analysis revealed a male karyotype with the presence of translocation between chromosome 15 and 17 [46 XY, t(15;17)(p11;q21)] in all metaphases, detectable at the level of banding resolution (ISCN) 400. Paroxysmal nocturnal hemoglobinuria clone was ruled out by flowcytometry on peripheral blood granulocytes. Prothrombin time and activated partial thromboplastin time were normal with no evidence of coagulopathy. He was given supportive treatment with packed red blood cell transfusion and started on oral cyclosporine 100 mg twice daily. Most cases of aplastic anemia are acquired and do not have any cytogenetic abnormalities though few patients have been described with these abnormalities at presentation ranging from 4% to 12%.[1–4] The relevance of cytogenetic abnormalities to the pathophysiology of AA is unknown in contrast to diseases like myelodysplastic syndrome and acute leukemias where diagnosis and treatment rests heavily on cytogenetic abnormalities. Both numerical and structural abnormalities have been reported in aplastic anemia. Numerical abnormalities include trisomies of chromosome 6, 7, 8, 13, 14 and 15 and monosomy 7 and 9 and structural abnormalities include t(3;11) and t(4;6).[1 2 4] Overall, the most common chromosomal abnormalities reported are trisomies of 6 and 8 and loss of chromosome 7.[1 4] Although unusual cytogenetics have been reported in patients with AA[5 6] including t(9;22) but t(15;17) has not been reported. The response to immunosuppressive therapy, durability of response, and progression to later clonal disorders in these patients did not appear to be different from patients with a normal karyotype though they might be at higher risk of progressing to myelodysplastic syndrome or acute myeloid leukemia.[2 4] Reciprocal translocation t(15;17)(q22;q21) is the characteristic abnormality found in 95% of patients with acute promyelocytic leukemia (APL) and most of them present with cytopenias.[7] Though our patient had chromosomal breakpoint 17q21 similar to that in APL, the breakpoint on chromosome 15 was different (i.e. at 15p11). The significance of this translocation in the pathogenesis of aplastic anemia is unknown as is true for other cytogenetic abnormalities reported, but we conclude that this novel cytogenetic translocation involving 17q21 may be involved in pathogenesis of cytopenias seen in this patient and probably in some patients with APL presenting with cytopenias.