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      Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees

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      1 , * , 2 , 3 , 4
      Nucleic Acids Research
      Oxford University Press

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          Abstract

          Interactive Tree Of Life ( http://itol.embl.de) is a web-based tool for the display, manipulation and annotation of phylogenetic trees. It is freely available and open to everyone. The current version was completely redesigned and rewritten, utilizing current web technologies for speedy and streamlined processing. Numerous new features were introduced and several new data types are now supported. Trees with up to 100,000 leaves can now be efficiently displayed. Full interactive control over precise positioning of various annotation features and an unlimited number of datasets allow the easy creation of complex tree visualizations. iTOL 3 is the first tool which supports direct visualization of the recently proposed phylogenetic placements format. Finally, iTOL's account system has been redesigned to simplify the management of trees in user-defined workspaces and projects, as it is heavily used and currently handles already more than 500,000 trees from more than 10,000 individual users.

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          Most cited references17

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          Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation.

          Interactive Tree Of Life (iTOL) is a web-based tool for the display, manipulation and annotation of phylogenetic trees. Trees can be interactively pruned and re-rooted. Various types of data such as genome sizes or protein domain repertoires can be mapped onto the tree. Export to several bitmap and vector graphics formats is supported. iTOL is available at http://itol.embl.de
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            Dendroscope 3: an interactive tool for rooted phylogenetic trees and networks.

            Dendroscope 3 is a new program for working with rooted phylogenetic trees and networks. It provides a number of methods for drawing and comparing rooted phylogenetic networks, and for computing them from rooted trees. The program can be used interactively or in command-line mode. The program is written in Java, use of the software is free, and installers for all 3 major operating systems can be downloaded from www.dendroscope.org. [Phylogenetic trees; phylogenetic networks; software.].
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              Performance, Accuracy, and Web Server for Evolutionary Placement of Short Sequence Reads under Maximum Likelihood

              We present an evolutionary placement algorithm (EPA) and a Web server for the rapid assignment of sequence fragments (short reads) to edges of a given phylogenetic tree under the maximum-likelihood model. The accuracy of the algorithm is evaluated on several real-world data sets and compared with placement by pair-wise sequence comparison, using edit distances and BLAST. We introduce a slow and accurate as well as a fast and less accurate placement algorithm. For the slow algorithm, we develop additional heuristic techniques that yield almost the same run times as the fast version with only a small loss of accuracy. When those additional heuristics are employed, the run time of the more accurate algorithm is comparable with that of a simple BLAST search for data sets with a high number of short query sequences. Moreover, the accuracy of the EPA is significantly higher, in particular when the sample of taxa in the reference topology is sparse or inadequate. Our algorithm, which has been integrated into RAxML, therefore provides an equally fast but more accurate alternative to BLAST for tree-based inference of the evolutionary origin and composition of short sequence reads. We are also actively developing a Web server that offers a freely available service for computing read placements on trees using the EPA.
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                Author and article information

                Journal
                Nucleic Acids Res
                Nucleic Acids Res
                nar
                nar
                Nucleic Acids Research
                Oxford University Press
                0305-1048
                1362-4962
                08 July 2016
                19 April 2016
                19 April 2016
                : 44
                : Web Server issue
                : W242-W245
                Affiliations
                [1 ]Biobyte solutions GmbH, Bothestr 142, 69126 Heidelberg, Germany
                [2 ]European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
                [3 ]Max Delbrück Centre for Molecular Medicine, 13125 Berlin, Germany
                [4 ]Department of Bioinformatics, Biocenter, University of Würzburg, 97074 Würzburg, Germany
                Author notes
                [* ]To whom correspondence should be addressed. Tel: +49 6221 3878534; Email: letunic@ 123456biobyte.de
                Author information
                http://orcid.org/0000-0003-3560-4288
                Article
                10.1093/nar/gkw290
                4987883
                27095192
                c26b3256-332e-4cb6-8536-d550995df2ea
                © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@ 123456oup.com

                History
                : 08 April 2016
                : 04 April 2016
                : 24 February 2016
                Page count
                Pages: 4
                Categories
                Web Server issue
                Custom metadata
                08 July 2016

                Genetics
                Genetics

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